The Drosophila protein Asp is involved in microtubule organization during spindle formation and cytokinesis (Articolo in rivista)

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Label
  • The Drosophila protein Asp is involved in microtubule organization during spindle formation and cytokinesis (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • Wakefield JG 1, Bonaccorsi S 2, Gatti M 1, 2. (2001)
    The Drosophila protein Asp is involved in microtubule organization during spindle formation and cytokinesis
    (literal)
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  • Wakefield JG 1, Bonaccorsi S 2, Gatti M 1, 2. (literal)
Pagina inizio
  • 637 (literal)
Pagina fine
  • 647 (literal)
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  • Asp is the first protein that has been shown to bind and stabilize the minus ends of the central spindle microtubules. Recent work has identified the human orthologue of Asp (ASPM). Mutations in the ASPM gene cause reduction in prenatal brain growth, resulting in microcephaly. James Wakefield was postdoctoral fellow in the framework of a EU TMR grant to M. Gatti. This paper is published in a highly valued journal with an impact factor of 12.52 in 2002. (literal)
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  • 153 (literal)
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  • M. Gatti a disposizione dell'IBPM (literal)
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  • Our results provide a model on the biological function of the Abnormal spindle protein (Asp). Asp acts similarly at the minus ends of microtubules of both the central spindle and the meiotic spindle pole. At the spindle poles Asp could bind and possibly cross-link the microtubule minus ends, ensuring their attachment to centrosomes after their release from these nucleating centers. Asp could also serve to cross-link the minus ends of central spindle microtubules, thus stabilizing this structure and allowing formation of the acto-myosin contractile apparatus required for cytokinesis. (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • 1. UNI Sapienza (Dipartimento Genetica Biologia Molecolare), 2. CNR (IBPM, ex Centro Genetica Evoluzionistica) (literal)
Titolo
  • The Drosophila protein Asp is involved in microtubule organization during spindle formation and cytokinesis (literal)
Abstract
  • Abnormal spindle (Asp) is a 220kD microtubule associated protein from Drosophila, that has been suggested to be involved in microtubule nucleation from the centrosome. Here we show that Asp is enriched at the poles of meiotic and mitotic spindles and localizes to the minus ends of central spindle microtubules. Localization to these structures is independent of a functional centrosome. Moreover, colchicine treatment disrupts Asp localization to the centrosome, indicating that Asp is not an integral centrosomal protein. In both meiotic and mitotic divisions of asp mutants, microtubule nucleation occurs from the centrosome and ? tubulin localizes correctly. However, spindle pole focusing and organization are severely affected. By examining cells that carry mutations both in asp and in asterless (asl), a gene required for centrosome function, we have determined the role of Asp in the absence of centrosomes. Phenotypic analysis of these double mutants shows that Asp is required for the aggregation of microtubules into focused spindle poles, reinforcing the conclusion that its function at the spindle poles is independent of any putative role in microtubule nucleation. Our data also suggest that Asp has a role in the formation of the central spindle. The inability of asp mutants to correctly organize the central spindle leads to disruption of the contractile ring machinery and failure in cytokinesis. (literal)
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