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Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells (Articolo in rivista)

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Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells (Articolo in rivista) (literal)

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Somma MP 1, Fasulo B 2, Cenci G 2, Cundari E 1, Gatti M 1,2. (2002)
Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells
in Molecular biology of the cell
(literal)

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This paper describes the first systematic analysis aimed at the identification of a specific class of mitotic genes using RNA interference in Drosophila tissue culture cells. The work, published in a highly valued journal with an impact factor of 7.6 in 2002, was highlighted in the Editors Choice of Science (Science, 296: 1571-1573, 2002). (literal)

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Although molecular genetic analyses in model systems have led to the discovery of many gene products involved in cytokinesis, it is clear that the inventory of cytokinetic proteins is still largely incomplete. We have shown that RNA interference (RNAi) in Drosophila tissue culture cells is a highly efficient method to identify genes required for cytokinesis. Our results predict that genome-wide studies using this method will permit identification of the majority of genes involved in Drosophila mitotic cytokinesis. (literal)

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1. CNR (IBPM, ex Centro di Genetica Evoluzionistica), 2. UNI Sapienza (Dipartimento Genetica e Biologia Molecolare) (literal)

Titolo

Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells (literal)

Abstract

We have used double strand RNA-mediated interference (RNAi) to study Drosophila cytokinesis. We show that dsRNAs for anillin (ani), acGAP, pavarotti (pav), rho1, pebble (pbl), spaghetti squash (sqh), syntaxin1A (syx1A) and twinstar (tsr) all disrupt cytokinesis in S2 tissue culture cells, causing gene-specific phenotypes. Our phenotypic analyses identify genes required for different aspects of cytokinesis, such as central spindle formation, actin accumulation at the cell equator, contractile ring assembly or disassembly and membrane behavior. Moreover, the cytological phenotypes elicited by RNAi reveal simultaneous disruption of multiple aspects of cytokinesis. These phenotypes suggest interactions between central spindle microtubules, the actin-based contractile ring and the plasma membrane, and lead us to propose that the central spindle and the contractile ring are interdependent structures. Finally, our results indicate that RNAi in S2 cells is a highly efficient method to detect cytokinetic genes, and predict that genome-wide studies using this method will permit identification of the majority of genes involved in Drosophila mitotic cytokinesis. (literal)

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