http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11365

Negative autoregulation of the Arabidopsis homeobox gene ATHB-2 (Articolo in rivista)

Type

Label

Negative autoregulation of the Arabidopsis homeobox gene ATHB-2 (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Ohgishi M. (1), Oka A. (1), Morelli G. (2), Ruberti I. (3), Aoyama T. (1) (2001)
Negative autoregulation of the Arabidopsis homeobox gene ATHB-2
in Plant journal (Print); Blackwell Scientific Publications, Oxford (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Ohgishi M. (1), Oka A. (1), Morelli G. (2), Ruberti I. (3), Aoyama T. (1) (literal)

Pagina inizio

Pagina fine

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

The paper demonstrates that ATHB-2, a key regulator of shade avoidance responses in Arabidopsis, has a negative autoregulatory loop. Negative feedback regulation is used by many organisms as an effective mechanisms for stabilizing the quantity of a substance. Assuming that the release of ATHB-2 molecules from the ATHB-2 promoter is the rate-limiting step for its gene expression, the concentration of ATHB-2 in the nuclei can be tightly controlled within a very narrow range in which the binding equivalence between ATHB-2 and the promoter is maintained. In addition, the autoregulatory loop consists of ATHB-2 alone, suggesting that the regulation is very rapid. This might contribute to rapid switching off of ATHB-2 expression when its induction signal stops. Autoregulation of ATHB-2 might provide both fine and rapid regulation of gene expression during shade avoidance responses. The paper is published in a valued international journal with an impact factor of 5.792. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

Rivista

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

In this paper, we performed a target-gene analysis using the HD-Zip-2-V-G system to investigate the interaction of ATHB-2 with its own gene and paralogues genes, since animal homeobox genes are frequently involved in autoregulatory networks. The results indicated that ATHB-2 has a negative autoregulatory loop and is possibly involved in a complicated regulatory network involving HD-Zip II subfamily genes. (literal)

Note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

(1) Institute for Chemical Research, Kyoto University; (2) Istituto Nazionale della Nutrizione (Unità di Nutrizione Sperimentale); (3) CNR (C.S. sugli Acidi Nucleici) (literal)

Titolo

Abstract

The Arabidopsis homeobox gene ATHB-2 is tightly regulated by light signals, and is thought to direct morphological changes during shade avoidance responses. To understand how ATHB-2 mediates light signals in plant morphogenesis, we investigated its transcriptional network. We constructed a gene encoding a chimeric transcription factor (HD-Zip-2-V-G) that is expected to activate target genes of ATHB-2 in a glucocorticoid-dependent manner. In transgenic Arabidopsis plants expressing HD-Zip-2-V-G, glucocorticoid treatment activates the ATHB-2 gene itself, independent of de novo protein synthesis. An in vitro DNase I-footprinting experiment showed that recombinant ATHB-2 protein specifically bound to an ATHB-2 promoter region. These complementary results indicate that ATHB-2 recognizes its own promoter. Consistent with the fact that ATHB-2 itself has been shown to act as a repressor, expression of the endogenous ATHB-2 gene was repressed in transgenic plants overexpressing an ATHB-2 transgene. Moreover, target-gene analysis using the HD-Zip-2-V-G suggested that ATHB-2 recognizes other HD-Zip II subfamily genes. We conclude that ATHB-2 has a negative autoregulatory loop and may be involved in a complicated transcriptional network involving paralogous genes, as in the case with animal homeobox genes. (literal)

Editore