http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11234
In Vivo Topography Of Rap1p-Dna Complex at S. Cerevisiae Tef2 Uasrpg During Transcriptional Regulation (Articolo in rivista)
- Type
- Label
- In Vivo Topography Of Rap1p-Dna Complex at S. Cerevisiae Tef2 Uasrpg During Transcriptional Regulation (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Alternative label
De Sanctis V., La Terra S., Bianchi A., Shore D., Burderi L., Di Mauro E., Negri R. (2002)
In Vivo Topography Of Rap1p-Dna Complex at S. Cerevisiae Tef2 Uasrpg During Transcriptional Regulation
in Journal of Molecular Biology
(literal)
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- De Sanctis V., La Terra S., Bianchi A., Shore D., Burderi L., Di Mauro E., Negri R. (literal)
- Pagina inizio
- Pagina fine
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- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- progetto di ricerca n. 17. Resp. Giovanna Costanzo (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Titolo
- In Vivo Topography Of Rap1p-Dna Complex at S. Cerevisiae Tef2 Uasrpg During Transcriptional Regulation (literal)
- Abstract
- We have analyzed in detail the structure of RAP1-UAS(RPG) complexes in
Saccharomyces cerevisiae cells using multi-hit KMnO(4), UV and micrococcal
nuclease high-resolution footprinting. Three copies of the Rap1 protein
are bound to the promoter simultaneously in exponentially growing cells,
as shown by KMnO(4) multi-hit footprinting analysis, causing extended and
diagnostic changes in the DNA structure of the region containing the UAS
(RPG). Amino acid starvation does not cause loss of Rap1p from the
complex; however, in vivo UV-footprinting reveals the occurrence of
structural modifications of the complex. Moreover, low-resolution
micrococcal nuclease digestion shows that the chromatin of the entire
region is devoid of positioned nucleosomes but is susceptible to changes
in accessibility to the nuclease upon amino acid starvation. The
implications of these results for the mechanism of Rap1p action are
discussed. (literal)
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