Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach (Articolo in rivista)

Type
Label
  • Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • A. Cariani; A. Piano; C. Consolandi; M. Severgnini; B. Castiglioni; G. Caredda; M. Candela; P. Serratore; G. De Bellis; F. Tinti (2012)
    Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach
    in International journal of food microbiology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • A. Cariani; A. Piano; C. Consolandi; M. Severgnini; B. Castiglioni; G. Caredda; M. Candela; P. Serratore; G. De Bellis; F. Tinti (literal)
Pagina inizio
  • 474 (literal)
Pagina fine
  • 482 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 153 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Articolo in cui si descrive lo sviluppo di una metodica microarray per l'identificazione di patogeni particolarmente pericolosi per la salute umana nei molluschi. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • AC, FT: Università di Bologna, Dipartimento di Biologia Evoluzionistica Sperimentale; AP, PS: Università di Bologna, Dipartimento. di Scienze Veterinarie; CC, MS, GC, GDB: ITB-CNR; BC: IBBA-CNR; MC: Università di Bologna, Dipartimento di Scienze Farmaceutiche. (literal)
Titolo
  • Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach (literal)
Abstract
  • Vibrios are a group of major foodborne pathogens widely distributed in marine environment. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the pathogenic species of Vibrio that pose the greatest threat to human health. However, other vibrios, e.g. Vibrio alginolyticus, Vibrio mimicus and Grimontia hollisae, apparently less relevant in the group of foodborne pathogens, have been sporadically found in outbreaks. For seafood safety and economic purposes, a rapid and powerful method for the specific identification of harmful Vibrio strains is needed. We developed a PCR-Ligase Detection Reaction-Universal Array (PCR-LDR-UA) assay for the simultaneous identification of pathogenic vibrios and detection of virulence coding genes. The entire procedure was validated on a total of 31 reference strains and isolates from clinical and environmental samples, as well as on bivalve tissue homogenates infected with different strains of target Vibrio species. Twenty-three shellfish samples directed to human consumption were successfully screened, thus demonstrating that the developed microarray-based platform could be a reliable and sensitive detection tool for the identification of harmful Vibrio strains in seafood. (literal)
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