A defective ABC transporter of the MRP family, responsible of the bean lpa1 mutation, affects the regulation of phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• A defective ABC transporter of the MRP family, responsible of the bean lpa1 mutation, affects the regulation of phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1111/j.1469-8137.2011.03666.x (literal)

Alternative label

• Panzeri D.; Cassani E.; Doria E.; Tagliabue G.; Forti L.; Campion B.; Bollini R.; Brearley C.; Pilu R.; Nielsen E.; Sparvoli F. (2011)
  A defective ABC transporter of the MRP family, responsible of the bean lpa1 mutation, affects the regulation of phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity
  in New phytologist (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Panzeri D.; Cassani E.; Doria E.; Tagliabue G.; Forti L.; Campion B.; Bollini R.; Brearley C.; Pilu R.; Nielsen E.; Sparvoli F. (literal)

Pagina inizio

• 70 (literal)

Pagina fine

• 93 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 191 (literal)

Rivista

• New phytologist (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• accattato, manuscript NPH-MS-2010-10944.R1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Panzeri D.,Tagliabue G.,Bollini R., Sparvoli F. IBBA-CNR Cassani E., Pilu R. Dipartimento di Produzione Vegetale, Universita` degli Studi di Milano, Italy Doria E., Nielsen E. Dipartimento di Genetica e Microbiologia, Universita` di Pavia, Pavia, Italy Forti L. Dipartimento di Chimica, Universita` di Modena, Modena, Italy Campion B. Unità di ricerca per l'Orticoltura CRA, Montanaso Lombardo, Lodi, Italy Brearley C. School of Biological Sciences, University of East Anglia, Norwich (literal)

Titolo

• A defective ABC transporter of the MRP family, responsible of the bean lpa1 mutation, affects the regulation of phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity (literal)

Abstract

• By screening an EMS mutagenised common bean population, we had previously identified the lpa (low phytic acid) 280-10 line that carries a monogenic recessive mutation conferring a 90% reduction of InsP6 content and, differently from most other lpa mutants, does not display negative pleiotropic effects associated to the mutation. In the present work we mapped the lpa-280-10 mutation on bean chromosome 1, in a region syntenic to soybean (Glycine max) chromosomes 19 and 3, where the lpa mutant CX1834 was previously mapped and correlated to multi-drug resistance protein (MRP) type ATP-binding cassette (ABC) transporter gene orthologous to Arabidopsis AtMRP5/AtABCC5 and maize ZmMRP4 (lpa1). Fine mapping of bean lpa-280-10 and DNA sequencing data showed the occurrence of a single amino acid substitution (E to K) in the orthologous PvMRP-1 gene. We also provide data on a second MRP gene (PvMRP-2) present both in soybean and bean genomes, which, despite the high sequence identity (90%) and a very similar pattern of expression during seed development to PvMRP-1, is not able to complement the lpa mutation, suggesting that it may have a different role in the plant. Transcriptional and metabolic analysis of phytic acid pathway demonstrated the existence of a negative feedback mechanism of InsP6, or its degradation products, acting on genes coding for key enzymes of phytic acid pathway and leading to a 30% reduction of myo-inositol. Our results show that the reduced content of myo-inositol is responsible of the ABA hypersensitive response found in lpa-280-10 seed germination, indicating a key role of this metabolite in the multiple pleiotropic effects often reported for lpa mutants. (literal)

Prodotto di

• Biotecnologie agro-industriali per il miglioramento genetico, il rispetto dell'ambiente e la tutela dei prodotti, la produzione di proteine e metaboliti di interesse alimentare, salutistico e farmacologico. (AG.P01.004.001) (Modulo)
• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Basi molecolari, fisiologiche e cellulari delle produzioni vegetali. (AG.P01.001.001) (Modulo)

Autore CNR

• Giovanni Tagliabue (Unità di personale esterno)
• DARIO PANZERI (Persona)
• FRANCESCA SPARVOLI (Persona)
• ROBERTO BOLLINI (Persona)

Insieme di parole chiave

• Parole chiave di "A defective ABC transporter of the MRP family, responsible of the bean lpa1 mutation, affects the regulation of phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Biotecnologie agro-industriali per il miglioramento genetico, il rispetto dell'ambiente e la tutela dei prodotti, la produzione di proteine e metaboliti di interesse alimentare, salutistico e farmacologico. (AG.P01.004.001) (Modulo)
• Basi molecolari, fisiologiche e cellulari delle produzioni vegetali. (AG.P01.001.001) (Modulo)

Autore CNR di

• FRANCESCA SPARVOLI (Persona)
• ROBERTO BOLLINI (Persona)
• Giovanni Tagliabue (Unità di personale esterno)
• DARIO PANZERI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• New phytologist (Rivista)

Insieme di parole chiave di

• Parole chiave di "A defective ABC transporter of the MRP family, responsible of the bean lpa1 mutation, affects the regulation of phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity" (Insieme di parole chiave)