http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11142

Differentially expressed genes associated with Staphylococcus aureus mastitis in dairy goats (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Differentially expressed genes associated with Staphylococcus aureus mastitis in dairy goats (Articolo in rivista) (literal)

Anno

2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.vetimm.2009.11.016 (literal)

Alternative label

Giuliano Pisoni; Paolo Moroni; Sam Genini; Alessandra Stella; Paul Boettcher; Paola Cremonesi; Licia Scaccabarozzi; Elisabetta Giuffra; Bianca Castiglioni (2010)
Differentially expressed genes associated with Staphylococcus aureus mastitis in dairy goats
in Veterinary immunology and immunopathology (Print); Academic Press Elsevier, Amsterdam (Paesi Bassi)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Giuliano Pisoni; Paolo Moroni; Sam Genini; Alessandra Stella; Paul Boettcher; Paola Cremonesi; Licia Scaccabarozzi; Elisabetta Giuffra; Bianca Castiglioni (literal)

Pagina inizio

208 (literal)

Pagina fine

217 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

135 (literal)

Rivista

Veterinary immunology and immunopathology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

doi:10.1016/j.vetimm.2009.11.016 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

Pubblicazione scientifica riguardante lo studio dellespressione genica della ghiandola mammaria caprina in corso di infezione con Staphylococcus aureus. (literal)

Note

PubMe (literal)
ISI Web of Science (WOS) (literal)
Google Scholar (literal)
Scopus (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Giuliano Pisoni, Paolo Moroni, Licia Scaccabarozzi - DIPAV-UNIMI, Milano; Alessandra Stella, Paul Boettcher, Paola Cremonesi, Bianca Castiglioni - IBBA-CNR, Lodi; Sam Genini, Elisabetta Giuffra - PTP, Lodi (literal)

Titolo

Differentially expressed genes associated with Staphylococcus aureus mastitis in dairy goats (literal)

Abstract

To study gene expression within the mammary glands of dairy goats with mastitis, mRNA was collected from milk somatic cells (MSCs) of left udder halves challenged with Staphylococcus aureus and right udder halves infused with PBS, as control, at different time points (0, 12, 24 and 48 h post-infection). Transcriptional profiles were investigated using bovine cDNA microarrays; of the total 288 differentially expressed genes identified with ANOVA analysis (False Discovery Rate = 0.05, 1.5-fold change), 26, 36 and 16 genes were down-regulated at 12, 24 and 48 h post-infection, respectively, while 60, 141 and 9 genes were up-regulated at the same corresponding time points. The expression profiles clearly changed at 24 h post-infection with 177 genes significantly altered, corresponding to a 10-fold increase of S. aureus bacterial count in milk from infected udders. Differential expression of selected genes (CD2BP2, BCAP31, MHCII, FOSL2, MAPK13, ILT5 and JUNB) was also confirmed by real-time PCR at the different time points considered, showing high correlation with the microarray measurements and high reliability of the microarray analyses. Themost readily inducible classes of genes in caprine MSCs infected with S. aureus were pro-inflammatory cytokines, chemokines and their receptors; IL-1a, lymphotoxin alpha, granulocyte chemotactic protein (CXCL6), and IL-2 receptor gamma were all upregulated in infected udders versus healthy controls. This study identified a number of differentially expressed genes induced by S. aureus intramammary infection and demonstrates the intricacy of the patterns of gene expression that influence host response to a complex pathogen of significant relevance to both human and veterinary medicine. (literal)

Editore