http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11129
Characterisation of structural genes involved in phytic acid biosynthesis in common bean (Phaseolus vulgaris L.) (Articolo in rivista)
- Type
- Label
- Characterisation of structural genes involved in phytic acid biosynthesis in common bean (Phaseolus vulgaris L.) (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s11032-009-9344-1 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Fileppi M.; Galasso I.; Tagliabue G.; Daminati M.G.; Campion B.; Doria E.; Sparvoli F. (literal)
- Pagina inizio
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- Rivista
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- accettato, Manuscript Number: MOLB1326R1
IF 2.272 (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Fileppi M.; Galasso I.; Tagliabue G.; Daminati M.G.; Sparvoli F. IBBA -CNR
Campion B. ISPORT- CRA, Montanaso Lombardo, LO
Doria E. Dipartimento di Genetia e Microbiologia, Università di Pavia (literal)
- Titolo
- Characterisation of structural genes involved in phytic acid biosynthesis in common bean (Phaseolus vulgaris L.) (literal)
- Abstract
- Abstract: Phytate (myo-inositol hexakisphosphate), the major form of phosphorous storage in plant seeds, is an inositol phosphate compound poorly digested by humans and monogastric animals. A major goal for grain crop improvement is the reduction of its content in the seed to improve micronutrient biovailabilty and phosphorus utilisation by humans and non-ruminant animals,respectively. We are interested in lowering phytic acid in common bean seed and to this goal we have undertaken a two-strategy approach: the isolation of mutants from an EMS mutagenised population (Campion et al., 2009) and the identification of genes coding for candidate enzymes involved in inositol phosphate metabolism for future targeted mutant isolation and/or study. In this paper we report data referred to the second approach and concerning the isolation and genomic organisation of Phaseolus vulgaris genes coding for myo-inositol 1-osphate synthase (PvMIPSs and PvMIPSv), inositol monophosphatase (PvIMP), myo-inositol kinase (PvMIK), inositol 1,4,5-tris-phosphate kinase (PvIPK2), inositol 1,3,4-triphosphate 5/6-kinase (PvITPKa and PvITPKa) and inositol 1,3,4,5,6 pentakisphosphate 2-kinase (PvIPK1). All these genes have been mapped on the common bean reference genetic map of McClean (NDSU) 2007 using a virtual mapping strategy. Bean markers, presumably associated to each gene of the phytic acid pathway, have also been identified. In addition, we provide a picture of the expression, during seed development, of the genes involved in phytic acid synthesis, including those such as MIK, IMP and IPK2, for which this information was lacking. (literal)
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