Parentage testing in alpacas (Vicugna pacos) using semi-automated fluorescent multiplex PCRs with 10 microsatellite markers. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Parentage testing in alpacas (Vicugna pacos) using semi-automated fluorescent multiplex PCRs with 10 microsatellite markers. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Agapito J., Rodríguez J., Herrera-Velit P., Timoteo O., Rojas P., Boettcher P.J., García F., Espinoza J.R.. (2008)
  Parentage testing in alpacas (Vicugna pacos) using semi-automated fluorescent multiplex PCRs with 10 microsatellite markers.
  in Animal genetics (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Agapito J., Rodríguez J., Herrera-Velit P., Timoteo O., Rojas P., Boettcher P.J., García F., Espinoza J.R.. (literal)

Pagina inizio

• 201 (literal)

Pagina fine

• 203 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 39 (literal)

Rivista

• Animal genetics (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Parentage testing in alpacas (Vicugna pacos) using semi-automated fluorescent multiplex PCRs with 10 microsatellite markers. (literal)

Abstract

• The aim of this study was to assess and apply a microsatellite multiplex system for parentage determination in alpacas. An approach for parentage testing based on 10 microsatellites was evaluated in a population of 329 unrelated alpacas from different geographical zones in Perú. All microsatellite markers, which amplified in two multiplex reactions, were highly polymorphic with a mean of 14.5 alleles per locus (six to 28 alleles per locus) and an average expected heterozygosity (H(E)) of 0.8185 (range of 0.698-0.946). The total parentage exclusion probability was 0.999456 for excluding a candidate parent from parentage of an arbitrary offspring, given only the genotype of the offspring, and 0.999991 for excluding a candidate parent from parentage of an arbitrary offspring, given the genotype of the offspring and the other parent. In a case test of parentage assignment, the microsatellite panel assigned 38 (from 45 cases) offspring parentage to 10 sires with LOD scores ranging from 2.19 x 10(+13) to 1.34 x 10(+15) and Delta values ranging from 2.80 x 10(+12) to 1.34 x 10(+15) with an estimated pedigree error rate of 15.5%. The performance of this multiplex panel of markers suggests that it will be useful in parentage testing of alpacas. (literal)

Prodotto di

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)

Autore CNR

• PAUL JOHN BOETTCHER (Unità di personale interno)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)

Autore CNR di

• PAUL JOHN BOETTCHER (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Animal genetics (Rivista)