Characterisation of arbuscular mycorrhizal fungi in roots by means of epifluorescence microscopy and molecular methods (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Characterisation of arbuscular mycorrhizal fungi in roots by means of epifluorescence microscopy and molecular methods (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Turrini A., Avio L., Bavila C. and Giovannetti M. (2008)
  Characterisation of arbuscular mycorrhizal fungi in roots by means of epifluorescence microscopy and molecular methods
  in Annals of microbiology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Turrini A., Avio L., Bavila C. and Giovannetti M. (literal)

Pagina inizio

• 157 (literal)

Pagina fine

• 162 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 58 (literal)

Rivista

• Annals of microbiology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• AL- IBBA-CNR, Milano; TA, BC, GM - Università di Pisa (literal)

Titolo

• Characterisation of arbuscular mycorrhizal fungi in roots by means of epifluorescence microscopy and molecular methods (literal)

Abstract

• In this work we characterised different species of the arbuscular mycorrhizal (AM) fungal genus Glomus by using Restriction Fragment Length Polymorphisms (RFLPs), combining epifluorescence microscopy and nested PCR, for the easy retrieval of AM fungal DNA in planta. Epifluorescence microscopy allowed us to select highly colonised root segments for DNA extraction, enhancing the chance of fungal DNA amplification by PCR. The primer pair used in nested PCR after a first reaction performed by using NS31/AM1 primers, showed a high specificity for Glomus DNA amplification and yielded enough fungal DNA for RFLP analyses. RFLP patterns of PCR products from colonised roots matched with reference profiles obtained from spores and discriminated the four Glomus species tested. The method described may be useful for checking the establishment of mycorrhizal colonisation by the inoculated fungal species in controlled experimental conditions (literal)

Prodotto di

• Basi molecolari, fisiologiche e cellulari delle produzioni vegetali. (AG.P01.001.001) (Modulo)
• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR

• LUCIANO AVIO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Characterisation of arbuscular mycorrhizal fungi in roots by means of epifluorescence microscopy and molecular methods" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Basi molecolari, fisiologiche e cellulari delle produzioni vegetali. (AG.P01.001.001) (Modulo)

Autore CNR di

• LUCIANO AVIO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Annals of microbiology (Rivista)

Insieme di parole chiave di

• Keywords of "Characterisation of arbuscular mycorrhizal fungi in roots by means of epifluorescence microscopy and molecular methods" (Insieme di parole chiave)