In transgenic rice, alpha- and beta-tubulin regulatory sequences control GUS amount and distribution through intron mediated enhancement and intron dependent spatial expression (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• In transgenic rice, alpha- and beta-tubulin regulatory sequences control GUS amount and distribution through intron mediated enhancement and intron dependent spatial expression (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s11248-008-9202-7 (literal)

Alternative label

• Gianì S.; Altana A.; Campanoni P.; Morello L.; Breviario D (2009)
  In transgenic rice, alpha- and beta-tubulin regulatory sequences control GUS amount and distribution through intron mediated enhancement and intron dependent spatial expression
  in Transgenic research
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Gianì S.; Altana A.; Campanoni P.; Morello L.; Breviario D (literal)

Pagina inizio

• 151 (literal)

Pagina fine

• 162 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Gli autori introducono per la prima volta nella comunità scientifica il termine IDSE per Intron-Dependent-Spatial-Expression (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.springerlink.com/content/g4855x71320n0820/?MUD=MP (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 18 (literal)

Rivista

• Transgenic research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Pubblicazione scientifica riguardante l’individuazione del ruolo degli introni nel controllo dell’espressione genica quantitativo, temporale e organo/tessuto specifico (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CP: Philip Morris Products SA, Neuchatel Switzerland GS, AA, ML, BD: Istituto Biologia e Biotecnologia Agraria-CNR, Milano Italy (literal)

Titolo

• In transgenic rice, alpha- and beta-tubulin regulatory sequences control GUS amount and distribution through intron mediated enhancement and intron dependent spatial expression (literal)

Abstract

• The genomic upstream sequence of the rice tubulin gene OsTub6 has been cloned, sequenced and characterized. The 52UTR sequence is interrupted by a 446 bp long leader intron. This feature is shared with two other rice ²-tubulin genes (OsTub4 and OsTub1) that, together with OsTub6, group in the same clade in the evolutionary phylogenetic tree of plant ²-tubulins. Similarly to OsTub4, the leader intron of OsTub6 is capable of sustaining intron mediated enhancement (IME) of gene expression, in transient expression assays. A general picture is drawn for three rice ±-tubulin and two rice ²-tubulin genes in which the first intron of the coding sequence for the formers and the intron present in the 52UTR for the latters, are important elements for controlling gene expression. We used OsTua2:GUS, OsTua3:GUS, OsTub4:GUS and OsTub6:GUS chimeric constructs to investigate the in vivo pattern of beta-glucuronidase (GUS) expression in transgenic rice plants. The influence of the regulatory introns on expression patterns was evaluated for two of them, OsTua2 and OsTub4. We have thus characterized distinct patterns of expression attributable to each tubulin isotype and we have shown that the presence of the regulatory intron can greatly influence both the amount and the actual site of expression. We propose the term Intron Dependent Spatial Expression (IDSE) to highlight this latter effect. (literal)

Prodotto di

• Biotecnologie agro-industriali per il miglioramento genetico, il rispetto dell'ambiente e la tutela dei prodotti, la produzione di proteine e metaboliti di interesse alimentare, salutistico e farmacologico. (AG.P01.004.001) (Modulo)
• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR

• DIEGO BREVIARIO (Unità di personale interno)
• ANDREA ALTANA (Persona)
• SILVIA GIANI' (Persona)
• LAURA EMMA MARIA MORELLO (Persona)

Insieme di parole chiave

• Keywords of "In transgenic rice, alpha- and beta-tubulin regulatory sequences control GUS amount and distribution through intron mediated enhancement and intron dependent spatial expression" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Biotecnologie agro-industriali per il miglioramento genetico, il rispetto dell'ambiente e la tutela dei prodotti, la produzione di proteine e metaboliti di interesse alimentare, salutistico e farmacologico. (AG.P01.004.001) (Modulo)

Autore CNR di

• LAURA EMMA MARIA MORELLO (Persona)
• DIEGO BREVIARIO (Unità di personale interno)
• SILVIA GIANI' (Persona)
• ANDREA ALTANA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Transgenic research (Rivista)

Insieme di parole chiave di

• Keywords of "In transgenic rice, alpha- and beta-tubulin regulatory sequences control GUS amount and distribution through intron mediated enhancement and intron dependent spatial expression" (Insieme di parole chiave)