Unexpected presence of Fagus orientalis complex in Italy as inferred from 45,000-year-old DNA pollen samples from Venice lagoon (Abstract/Poster in convegno)

Type
Label
  • Unexpected presence of Fagus orientalis complex in Italy as inferred from 45,000-year-old DNA pollen samples from Venice lagoon (Abstract/Poster in convegno) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Donatella Paffetti, Cristina Vettori, David Caramelli, Cristiano Vernesi, Martina Lari, Arturo Paganelli, Ladislav Paule and Raffaello Giannini (2007)
    Unexpected presence of Fagus orientalis complex in Italy as inferred from 45,000-year-old DNA pollen samples from Venice lagoon
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Donatella Paffetti, Cristina Vettori, David Caramelli, Cristiano Vernesi, Martina Lari, Arturo Paganelli, Ladislav Paule and Raffaello Giannini (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Results: 86 populations from 4 Western Euroasistic taxa were sampled, and sequenced for the trnL-trnF region to verify the efficiency of this cpDNA region in identifying the Fagus spp.. Furthermore, Fagus crenata (2 populations), Fagus grandifolia (2 populations), Fagus japonica, Fagus hayatae, Quercus species and Castanea species were analysed to better resolve the phylogenetic inference. Our results show that this cpDNA region harbour some informative sites that allow to infer relationships among the species within the Fagaceae family. In particular, few specific and fixed mutations were able to discriminate and identify all the different Fagus species. Considering a short fragment of 176 base pairs within the trnL intron, 2 transversions were found able in distinguishing the F. orientalis complex taxa (F. orientalis, F. taurica and F. moesiaca) from the remaining Fagus spp. (F. sylvatica, F. japonica, F. hayataea, F. crenata and F. grandifolia). This permits to analyse this fragment also in ancient samples, where DNA is usually highly degraded. The sequences data indicate that the DNA recovered from ancient pollen belongs to the F. orientalis complex since it displays the informative sites characteristic of this complex. (literal)
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  • Background: Phylogeographic analyses on the Western Euroasiatic Fagus taxa (F. orientalis, F. sylvatica, F. taurica and F. moesiaca) is available, however, the subdivision of Fagus spp. is unresolved and there is no consensus on the phylogeny and on the identification (both with morphological than molecular markers) of Fagus Eurasiatic taxa. For the first time molecular analyses of ancient pollen, dated at least 45,000 years ago, were used in combination with the phylogeny analysis on current species, to identify the Fagus spp. present during the Last Interglacial period in Italy. In this work we aim at testing if the trnL-trnF chloroplast DNA (cpDNA) region, that has been previously proved efficient in discriminating different Quercus taxa, can be employed in distinguishing the Fagus species and in identifying the ancient pollen. (literal)
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  • Department of Environmental and Forestry Technologies and Sciences, University of Florence, Via San Bonaventura 13, 50145 Florence, Italy, Plant Genetics Institute, National Research Council, Via Madonna del Piano 10, 50019, Sesto Fiorentino (FI), Italy, Dipartimento di Biologia Animale e Genetica, Laboratorio di Antropologia, University of Florence, Via del Proconsolo 12, 50122 Firenze, Italy, Centro Ecologia Alpina, Viote del Monte Bondone, 38040 Trento, Italy, Department of Biology, University of Padova, Via U. Bassi 58/B, I-35121, Padova, Italy Faculty of Forestry, Technical University, SK-96053 Zvolen, Slovakia (literal)
Titolo
  • Unexpected presence of Fagus orientalis complex in Italy as inferred from 45,000-year-old DNA pollen samples from Venice lagoon (literal)
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