Development of a single nucleotide polymorphism genotyping microarray platform for the identification of bovine milk protein genetic polymorphisms. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Development of a single nucleotide polymorphism genotyping microarray platform for the identification of bovine milk protein genetic polymorphisms. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Stefania Chessa; Francesca Chiatti; Gabriella Ceriotti; Anna Caroli; Clarissa Consolandi; Giulio Pagnacco; Bianca Castiglioni (2007)
  Development of a single nucleotide polymorphism genotyping microarray platform for the identification of bovine milk protein genetic polymorphisms.
  in Journal of dairy science; American Dairy Science Association-ADSA, Champaign (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Stefania Chessa; Francesca Chiatti; Gabriella Ceriotti; Anna Caroli; Clarissa Consolandi; Giulio Pagnacco; Bianca Castiglioni (literal)

Pagina inizio

• 451 (literal)

Pagina fine

• 464 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 90 (literal)

Rivista

• Journal of dairy science (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 14 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Pubblicazione scientifica riguardante lo sviluppo di un microarray diagnostico per la genotipizzazione delle lattoproteine bovine (literal)

Note

• PubMe (literal)
• Scopu (literal)
• Google Scholar (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Stefania Chessa, Francesca Chiatti, Gabriella Ceriotti, Giulio Pagnacco - Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Universita degli Studi di Milano, Via Trentacoste 2, Milano 20134, Italy; Anna Caroli - Dipartimento di Scienze Biomediche e Biotecnologie, Universita degli Studi di Brescia, Viale Europa 11, Brescia 25123, Italy; Clarissa Consolandi - Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Via Fratelli Cervi 93, Segrate 20090, Italy; Bianca Castiglioni - Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Via Bassini 15, Milano 20133, Italy (literal)

Titolo

• Development of a single nucleotide polymorphism genotyping microarray platform for the identification of bovine milk protein genetic polymorphisms. (literal)

Abstract

• The objective of this study was to develop and validate a fast method for typing the main mutations of bovine milk protein genes by using microarray technology. An approach based on Ligation Detection Reaction (LDR) and a Universal Array (UA) was used. Polymorphisms both in the coding and non coding sequences of αS1-casein, β-casein, κ-casein, and β-lactoglobulin genes were considered due to their well known effects on milk composition and cheese-making attitude. A total of 22 polymorphic sites, corresponding to 21 different variants, were included in the diagnostic microarray. First, a multiplex PCR (mPCR) was developed in order to amplify simultaneously all the DNA target sequences. Secondly, the LDR/UA assay was implemented. The method was validated by analyzing 100 Italian Friesian DNA samples, which were also genotyped by conventional methods both at the protein level by means of milk isoelectrofocusing and at the molecular level using PCR-RFLP and PCR-Single Strand Conformation Polymorphisms (SSCP) techniques. The genotypes obtained using the LDR/UA approach were in full agreement with those obtained by the conventional analyses. An important result of the LDR/UA assay was a more accurate genotyping of the different milk protein alleles than conventional typing methods. At the ê-casein gene, in fact, four samples were heterozygous for an allele coding for Thr136 and Ala148. Such variant, which can be considered as the wild type of the genus Bos, is not usually identifiable by the conventional typing methods used. The mPCR/LDR/UA approach developed provides for an accurate, inexpensive and high-throughput assay that does not exhibit false positive or false negative signals, thus making it highly suitable for the animal genotyping. (literal)

Editore

• American Dairy Science Association-ADSA (Editore)

Prodotto di

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)

Autore CNR

• CLARISSA CONSOLANDI (Persona)
• STEFANIA CHESSA (Persona)
• GIULIO GIORGIO ALESSANDRO PAGNACCO (Unità di personale esterno)
• BIANCA MARIA ELISABETTA CASTIGLIONI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Development of a single nucleotide polymorphism genotyping microarray platform for the identification of bovine milk protein genetic polymorphisms." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)

Autore CNR di

• CLARISSA CONSOLANDI (Persona)
• BIANCA MARIA ELISABETTA CASTIGLIONI (Unità di personale interno)
• GIULIO GIORGIO ALESSANDRO PAGNACCO (Unità di personale esterno)
• STEFANIA CHESSA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of dairy science (Rivista)

Editore di

• American Dairy Science Association-ADSA (Editore)

Insieme di parole chiave di

• Keywords of "Development of a single nucleotide polymorphism genotyping microarray platform for the identification of bovine milk protein genetic polymorphisms." (Insieme di parole chiave)