Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota (Articolo in rivista)

Type
Label
  • Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Albani D., Giorgetti L., Pitto L., Luo M., Cantoni R.M., Erra Pujada M., Rotino G.L., Cella R. (2005)
    Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota
    in European journal of histochemistry (Testo stamp.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Albani D., Giorgetti L., Pitto L., Luo M., Cantoni R.M., Erra Pujada M., Rotino G.L., Cella R. (literal)
Pagina inizio
  • 107 (literal)
Pagina fine
  • 116 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 49 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ Pavia, Dipartimento Genet & Microbiol, I-27100 Pavia, Italy Univ Sassari, Dipartimento Bot & Ecol Vegetale, I-07100 Sassari, Italy CNR, IBBA, I-56100 Pisa, Italy Ist Sperimentale Orticoltura, Sez Montanaso L, Montanaso Lombardo, LO, Italy (literal)
Titolo
  • Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota (literal)
Abstract
  • The pattern of expression of a carrot dhfr-ts gene was evaluated in different plant organs, in somatic embryos, and in hypocotyl explants induced to dedifferentiate in vitro by the addition of the synthetic auxin 2,4 dichorophenoxyacetic acid. The promoter of this gene was also placed upstream of a uidA (GUS) reporter gene and, using biolistic and protoplasts transient expression assays, was shown to drive a particularly high level of expression in actively growing suspension cells. The results from these expression analyses combined with the presence of putative cell cycle-related cis-acting elements in the dhfr-ts promoter, strongly point to a cell division-dependent expression of this gene. (literal)
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