http://www.cnr.it/ontology/cnr/individuo/prodotto/ID11001
Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota (Articolo in rivista)
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- Label
- Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Alternative label
Albani D., Giorgetti L., Pitto L., Luo M., Cantoni R.M., Erra Pujada M., Rotino G.L., Cella R. (2005)
Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota
in European journal of histochemistry (Testo stamp.)
(literal)
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- Albani D., Giorgetti L., Pitto L., Luo M., Cantoni R.M., Erra Pujada M., Rotino G.L., Cella R. (literal)
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- ISI Web of Science (WOS) (literal)
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- Univ Pavia, Dipartimento Genet & Microbiol, I-27100 Pavia, Italy
Univ Sassari, Dipartimento Bot & Ecol Vegetale, I-07100 Sassari, Italy
CNR, IBBA, I-56100 Pisa, Italy
Ist Sperimentale Orticoltura, Sez Montanaso L, Montanaso Lombardo, LO, Italy (literal)
- Titolo
- Proliferation-dependent pattern of expression of a dihydrofolate reductase-thymidylate synthase gene from Daucus carota (literal)
- Abstract
- The pattern of expression of a carrot dhfr-ts gene was evaluated in different plant organs, in somatic embryos, and in hypocotyl explants induced to dedifferentiate in vitro by the addition of the synthetic auxin 2,4 dichorophenoxyacetic acid. The promoter of this gene was also placed upstream of a uidA (GUS) reporter gene and, using biolistic and protoplasts transient expression assays, was shown to drive a particularly high level of expression in actively growing suspension cells. The results from these expression analyses combined with the presence of putative cell cycle-related cis-acting elements in the dhfr-ts promoter, strongly point to a cell division-dependent expression of this gene. (literal)
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