http://www.cnr.it/ontology/cnr/individuo/prodotto/ID109266

Sequencing and cardiac expression of natriuretic peptide receptors A and C in normal and heart failure pigs (Abstract/Comunicazione in atti di convegno)

Type

Prodotto della ricerca (Classe)
Abstract/Comunicazione in atti di convegno (Classe)

Label

Sequencing and cardiac expression of natriuretic peptide receptors A and C in normal and heart failure pigs (Abstract/Comunicazione in atti di convegno) (literal)

Anno

2010-01-01T00:00:00+01:00 (literal)

Alternative label

Cabiati M.; Campan M.; Caselli C.; Prescimone T.; Giannessi D.; Del Ry S. (2010)
Sequencing and cardiac expression of natriuretic peptide receptors A and C in normal and heart failure pigs
in Heart Failure Congress, Berlin
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Cabiati M.; Campan M.; Caselli C.; Prescimone T.; Giannessi D.; Del Ry S. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

In: HF Congress - Heart Failure Congress (Berlin, 29 May - 01 June 2010). Abstract, vol. Supplements 9 article n. S168. European Journal of Heart Failure, 2010. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

Pourpose: Natriuretic peptide receptors (NPRs) are capable to inhibit cardiac remodelling in heart failure (HF) targeted by novel pharmacological treatments, but their sequencing is still missing in minipig, the elective experimental model of HF. The pig constitutes an animal model largely used in experimental pathology owing of its similarity with human anatomo-physiology but Sus Scrofa genoma is not completely sequenced. Aim: to complete the characterization of NPR system in Sus Scrofa, sequencing NPR-A and NPR-C and to evaluate NPRs mRNA expression in experimental minipig HF models. Methods: to sequence NPR-A and NPR-C gene in Sus Scrofa we exploited the high homology between species, in particular between human and pig. For this reason, the optimal conditions were obtained using human cardiac tissue deriving from left ventricle (LV) of patients undergoing heart transplantation. ANP, BNP, NPR-A and NPR-C mRNA expressions were evaluated using cardiac tissue samples collected from adult male minipigs (weight 35-40 kg) without (control, n=4) and with pacing-induced HF (n=5). Results: Pig NPR-A and NPR-C mRNA were sequenced and resulted in 179 and 203 bp, respectively (GenBank accession n°: FJ518622, FJ518621 respectively). Compared to control, ANP and BNP gene expression resulted higher in all the cardiac chambers of HF heart. This increase is associated to a down-regulation of NPR-A and an up-regulation of NPR-C in HF. Conclusions: NPR-A and NPR-C gene in Sus Scrofa were sequenced and their mRNA expression were characterized simultaneously in all cardiac chambers of normal and HF minipigs. These sequences provide a new tool to investigate the role of NPs system under physiological and pathological conditions and their response to therapeutic interventions. (literal)

Note