A dual effect on protein synthesis and degradation modulates tubulin level in rice cells treated with oryzalin. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A dual effect on protein synthesis and degradation modulates tubulin level in rice cells treated with oryzalin. (Articolo in rivista) (literal)

Anno

• 2002-01-01T00:00:00+01:00 (literal)

Alternative label

• Gianì S. 1, Campanoni P. 1, and Breviario D. 1 (2002)
  A dual effect on protein synthesis and degradation modulates tubulin level in rice cells treated with oryzalin.
  in Planta
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Gianì S. 1, Campanoni P. 1, and Breviario D. 1 (literal)

Pagina inizio

• 837 (literal)

Pagina fine

• 847 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Gli erbicidi usati sono innocui per le tubuline di origine animale ma sono efficaci contro quelle dei protozoi. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 214 (literal)

Rivista

• Planta (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Pubblicazione nel triennio 2001-2003 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Pubblicazione scientifica pertinente alle attività in corso riguardanti il ruolo delle tubuline nella crescita cellulare ed i meccanismi che ne controllano l'espressione in presenza di erbicidi ad attività antitmitotica. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 CNR (IBBA) (literal)

Titolo

• A dual effect on protein synthesis and degradation modulates tubulin level in rice cells treated with oryzalin. (literal)

Abstract

• The effect of the anti-microtubular drug oryzalin on growth and morphology of cultured rice (Oryza sativa L., cv. Roncarolo) cells was evaluated with specific reference to mechanisms that control intracellular tubulin levels. The addition of oryzalin caused a great reduction in the level of both alpha- and beta-tubulin polypeptides, as detected by Western blot analysis. However, no appreciable decrease was observed in the population of total or isotype-specific alpha- and beta-tubulin mRNAs. Only within the first 24 h of the oryzalin treatment, when the level of both alpha- and beta-tubulin polypeptides was still undiminished, was a consistent reduction in the amount of total beta-tubulin mRNA observed. Pulse-chase experiments performed on rice cells grown in the presence of 1 microM oryzalin revealed the presence of two distinct mechanisms that negatively control alpha- and beta-tubulin polypeptide levels. (i) There was an immediate effect on protein synthesis, which resulted in a reduction in the level of newly synthesized tubulin. (ii) There was a delayed response characterized by a substantial degradation of both alpha- and beta-tubulin monomers; this degradation occurred after 24 h of herbicide treatment. The possible involvement of Ca2+ in the degradation of the unincorporated tubulin monomers is also documented and discussed. (literal)

Prodotto di

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR

• SILVIA GIANI' (Persona)
• DIEGO BREVIARIO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "A dual effect on protein synthesis and degradation modulates tubulin level in rice cells treated with oryzalin." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR di

• DIEGO BREVIARIO (Unità di personale interno)
• SILVIA GIANI' (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Planta (Rivista)

Insieme di parole chiave di

• Parole chiave di "A dual effect on protein synthesis and degradation modulates tubulin level in rice cells treated with oryzalin." (Insieme di parole chiave)