http://www.cnr.it/ontology/cnr/individuo/prodotto/ID109204

Cardiac metabolism with hyperpolarized [1-13C]pyruvate: a feasibility study in mini-pig with a large dose injection (Abstract/Poster in atti di convegno)

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Abstract/Poster in atti di convegno (Classe)
Prodotto della ricerca (Classe)

Label

Cardiac metabolism with hyperpolarized [1-13C]pyruvate: a feasibility study in mini-pig with a large dose injection (Abstract/Poster in atti di convegno) (literal)

Anno

2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1186/1532-429X-12-S1-T7 (literal)

Alternative label

Menichetti L.; Frijia F.; Lionetti V.; Santarelli M. F.; Ardenkjaer-Larsen J. H.; Giovannetti G.; Positano V.; De Marchi D.; Aquaro G.; Campan E.; Hartwig V.; Milanesi M.; Recchia F. A.; Landini L.; Lombardi M. (2010)
Cardiac metabolism with hyperpolarized [1-13C]pyruvate: a feasibility study in mini-pig with a large dose injection
in 13th Annual SCMR Scientific Sessions - 2010, Phoenix, AZ, USA, 21-24 January 2010
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Menichetti L.; Frijia F.; Lionetti V.; Santarelli M. F.; Ardenkjaer-Larsen J. H.; Giovannetti G.; Positano V.; De Marchi D.; Aquaro G.; Campan E.; Hartwig V.; Milanesi M.; Recchia F. A.; Landini L.; Lombardi M. (literal)

Pagina inizio

232 (literal)

Pagina fine

233 (literal)

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http://dx.doi.org/10.1186/1532-429X-12-S1-T7 (literal)

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12 (literal)

Rivista

Journal of cardiovascular magnetic resonance (Rivista)

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In: Journal of Cardiovascular Magnetic Resonance. Abstract, vol. 12 (supp.1) article n. T7. Biomed Central, 2010. (literal)

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2 (literal)

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S1 (literal)

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Introduction MRI with hyperpolarised 13C represents a promising modality for in vivo spectroscopy and it could provide a unique opportunity for non invasive assessment of cardiac regional metabolism. Purpose The aim of this work is to study real time in vivo cardiac metabolism after intravenous (i.v.) injection of hyperpolarized [113C]-pyruvate in a large animal model with a clinical 3T scanner. (literal)

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Abstract (literal)
Google Scholar (literal)

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CNR-IFC, Pisa, Fondazione G. Monasterio CNR-Regione Toscana, Pisa, Italy, Scuola Superiore Sant'Anna, Pisa (Italy), Sector of Medicine, General Electric Healthcare, Denmark, Department of Physiology, New York Medical College, University of Pisa (literal)

Titolo

Cardiac metabolism with hyperpolarized [1-13C]pyruvate: a feasibility study in mini-pig with a large dose injection (literal)

Abstract

Introduction MRI with hyperpolarised 13C represents a promising modality for in vivo spectroscopy and it could provide a unique opportunity for non invasive assessment of cardiac regional metabolism. Purpose The aim of this work is to study real time in vivo cardiac metabolism after intravenous (i.v.) injection of hyperpolarized [113C]-pyruvate in a large animal model with a clinical 3T scanner. Methods Animal model Four normal male mini-pigs (35 kg) were maintained under deep sedation with midazolam (0.1 mg/kg/h i.v.) while ECG, temperature and arterial blood pressure were monitored (SA Instruments, New York USA ). Polarization 13C-1-pyruvate Hyperpolarization was performed using Dynamic Nuclear Polarization (Hypersense, Oxford Instruments, Oxford, UK). The final injection solution contained 230 mM sodium [1-13C]pyruvate, 100 mM TRIS buffer, 0.27 mM Na2EDTA and 20 microM Dotarem (Guerbet, Paris, France). Temperature of solution was about 37ºC and pH = 7.6. A dose of 20 mL was administered over 10 s by manual injection. MR studies The mini-pigs underwent both 1H MR imaging and hyperpolarized 13C MRS. The experiments were performed with a 3 T GE Signa HDx (GE Healthcare, Waukesha, WI, USA) scanner with a 13C quadrature birdcage coil (Rapid Biomedical, Würzburg, Germany). Anatomical imaging was acquired with the body coil and FIESTA sequence (FOV=35, FA=45, TE/TR=1.71ms/3.849ms). 13C dynamic spectra were acquired using elliptic-FIDCSI pulse sequence (bandwidth 5000Hz, 2048 pts, 10º FA). A long-axis slice of 20 mm was selected during excitation. Spectra covering the heart were acquired from the beginning of the injection of the hyperpolarized [1-13C]pyruvate, every 2 s, for 120 s. Data processing Data processing was performed by using MATLAB (The Mathworks, Inc., Natick, MA) and jMRUI software tools. The dynamic spectra were phase corrected by adjusting both zero-and first-order frequency-dependent phase components. Pyruvate, pyruvate hydrate, alanine, lactate, and bicarbonate were estimated by using AMARES algorithm included into jMRUI tools. Accordingly, time courses of metabolites are generated. Results and discussion Spectra obtained from the dynamic acquisition are shown in Figure 1. The [1-13C]pyruvate, [1-13C]pyruvate hydrate and metabolites peaks ([1-13C]lactate, [1-13C]alanine, and 13C-bicarbonate) have been detected and plotted. Figure 2 shows the in vivo time course of cardiac metabolites. Conclusions Imaging cardiac metabolism with hyperpolarized 13C is feasible with 3T MRI in an animal model that closely resembles the human heart phenotype (literal)

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