http://www.cnr.it/ontology/cnr/individuo/prodotto/ID109167
Fibrin scaffolds seeded with endothelial progenitor cells for tissue engineering applications (Abstract/Poster in atti di convegno)
- Type
- Label
- Fibrin scaffolds seeded with endothelial progenitor cells for tissue engineering applications (Abstract/Poster in atti di convegno) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Alternative label
Magera A.; Barsotti M. C.; Lemmi M.; Armani C.; Arici R.; Iorio M. C.; Soldani G.; Balbarini A.; Di Stefano R. (2008)
Fibrin scaffolds seeded with endothelial progenitor cells for tissue engineering applications
in 2° Forum Nazionale dei Giovani Ricercatori su Materiali Polimerici e Biomateriali, Genova
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Magera A.; Barsotti M. C.; Lemmi M.; Armani C.; Arici R.; Iorio M. C.; Soldani G.; Balbarini A.; Di Stefano R. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- In: 2° Forum Nazionale dei Giovani Ricercatori su Materiali Polimerici e Biomateriali (Genova, 4-5 luglio 2008). Abstract, pp. 1 - 1. Forum Nazionale dei Giovani Ricercatori su Materiali Polimerici e Biomateriali, 2008. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
- Purpose To evaluate the use of fibrin as alternative biological scaffold for the in vitro culture of endothelial progenitor cells (EPC) Methods Fibrinogen (F, 4.5-36 mg/ml) and thrombin (T, 12.5-50 U/ml) were mixed to obtain the fibrin matrix and analysed by scanning electron microscopy (SEM and CRYO-SEM). EPC were obtained from human peripheral blood mononuclear cells and cultured for 1 week on the fibrin scaffolds at the concentration of 1 106 cells/cm2 in endothelial growth medium. As a control, fibronectin coating was used. After 1 week, both cell viability (calcein AM) and metabolic activity (WST-1) were measured. The expression of endothelial markers was evaluated by flow cytometry and Real Time RT PCR. The expression of the transcription factors Nanog and Oct 4, playing key roles in self-renewal, was also assessed. Results. Analysis of SEM pictures showed fibre diameters of 117-177 nm, with a trend to an increase when fibrinogen is lower, and fibre density of 88-96 %, while CRYO-SEM showed a porous fibrin structure in a nanometer and micrometer scale. Cells viability at 1 week was not significantly affected by fibrin (95% viable cells), whereas the metabolic activity was higher on fibrin. Fibrin matrix gave endothelial differentiation as well as fibronectin as confirmed by the expression of endothelial markers. However, higher levels of Nanog mRNA expression was detected on fibrin than on fibronectin matrix. Conclusions Fibrin has the potential to be used as biodegradable scaffold due to the presence of a nanometer and micrometer porous structure. (literal)
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Cardio-Thoracic Department, University Hospital, Pi, Cardio-Thoracic Department, University Hospital, Pisa, Dipartimento Cardiotoracico, Università di Pisa, IFC-CNR - Fondazione G. Monasterio, Massa Carrara (literal)
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- Fibrin scaffolds seeded with endothelial progenitor cells for tissue engineering applications (literal)
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