Alternative splicing of NEH-1 mediates Na-Li countertransport and associate with activity rate (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Alternative splicing of NEH-1 mediates Na-Li countertransport and associate with activity rate (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Alternative label

• Zerbini G., Maestroni A., Breviario D., Mangili R., Casari G. (2003)
  Alternative splicing of NEH-1 mediates Na-Li countertransport and associate with activity rate
  in Diabetes (N.Y.N.Y.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Zerbini G., Maestroni A., Breviario D., Mangili R., Casari G. (literal)

Pagina inizio

• 1511 (literal)

Pagina fine

• 1518 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 52 (literal)

Rivista

• Diabetes (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Alternative splicing of NEH-1 mediates Na-Li countertransport and associate with activity rate (literal)

Abstract

• Sodium-lithium countertransport (SLC) is an ouabain-insensitive exchange of Na for Li found in the erythrocyte membrane of several mammalian species. Although increased SLC activity is presently the most consistent intermediate phenotype of essential hypertension and diabetic nephropathy in humans, the gene responsible for this membrane transport has not been identified. Because of functional similarities, SLC was suggested to represent an in vitro mode of operation of the Na-H exchanger (NHE). This hypothesis, however, has been long hampered by the total insensitivity of SLC to amiloride, which is an intrinsic inhibitor of the first isoform of NHE, the only NHE isoform detected in human erythrocytes. We describe here the identification in human reticulocytes and erythrocytes of an alternative splicing of NHE lacking the amiloride binding site. Transfection experiments with this spliced variant restore amiloride-insensitive, phloretin-sensitive SLC activity. Expression of both regular and spliced transcripts of NHE is increased in subjects with high SLC activity. Altogether, these findings, by extending to NHE the characteristics of inheritance and predictivity previously attributed to SLC, eventually restore the candidacy of NHE isoform 1 as a gene involved in the pathogenesis of essential hypertension and diabetic nephropathy. (literal)

Prodotto di

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR

• DIEGO BREVIARIO (Persona)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR di

• DIEGO BREVIARIO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Diabetes (Rivista)