The omega-3 fatty acid docosahexaenoate (DHA) reduces cyclooxygenase(COX)-2 induction by inhibiting NADP(H) oxidase and PKCe: possible involvement of 15-lipoxigenase(LO)-1 (Abstract in rivista)

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  • The omega-3 fatty acid docosahexaenoate (DHA) reduces cyclooxygenase(COX)-2 induction by inhibiting NADP(H) oxidase and PKCe: possible involvement of 15-lipoxigenase(LO)-1 (Abstract in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Massaro M.; Scoditti E.; Carluccio M. A.; Storelli C.; Distante A.; De Caterina R. (2007)
    The omega-3 fatty acid docosahexaenoate (DHA) reduces cyclooxygenase(COX)-2 induction by inhibiting NADP(H) oxidase and PKCe: possible involvement of 15-lipoxigenase(LO)-1
    in European Heart Journal, 2007
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Massaro M.; Scoditti E.; Carluccio M. A.; Storelli C.; Distante A.; De Caterina R. (literal)
Convegno
  • European Heart Journal (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • In: European Heart Journal, vol. 28 pp. 281 - 281. Oxord Univerity Press, 2007. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • A high intake of the omega-3 fatty acids has been associated with systemic anti-inflammatory effects and cardiovascular protection, but the molecular basis responsible for these effects remains incompletely defined. COX-2 is a proinflammatory enzyme the expression of which is increased in human atheroma. We here investigated the effects of DHA on COX-2 expression in human saphenous vein endothelial cells (HSVEC) challenged with proinflammatory stimuli and explored the underlying molecular mechanisms. Methods and Results: A long-term (24-72 h) exposure of HSVEC to DHA (10-25 mmol/L) significantly reduced COX-2 protein (by 50%, at Western analysis) and mRNA (by 70%, at Northern analysis) as induced by IL1±, through the attenuation of NF-kB activation (by 60%, at EMSA). Since protein kinase C (PKC), and NAD(P)H oxidase all participate in IL-1-mediated COX-2 expression, we tested whether these enzymatic activities were affected by DHA. Western analysis showed that DHA reduces the activation of NAD(P)H oxidase and PKC-e but not -± or -¶. To address the question whether DHA itself or DHA-derived products were responsible for these effects, we inhibited (by pharmacological and molecular approaches, siRNA) the most important enzymes involved in polyunsaturated fatty acid metabolism, showing that only the 15-LO-1 inhibition partially reverted the DHA inhibitory effect on COX-2 expression (by 30%, at Western analysis), thus suggesting that the 15-LO-1 products mediate part of DHA effects. These data provide a mechanistic basis for antiinflammatory and possibly plaque-stabilizing effects of DHA. (literal)
Titolo
  • The omega-3 fatty acid docosahexaenoate (DHA) reduces cyclooxygenase(COX)-2 induction by inhibiting NADP(H) oxidase and PKCe: possible involvement of 15-lipoxigenase(LO)-1 (literal)
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