Matrix metalloprotease-2 and -9 concentration and activity in culture medium and serum samples: methodological evaluation of two different methods (Abstract in rivista)

Type

• Prodotto della ricerca (Classe)
• Abstract in rivista (Classe)

Label

• Matrix metalloprotease-2 and -9 concentration and activity in culture medium and serum samples: methodological evaluation of two different methods (Abstract in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Colotti C.; Angeli V.; Del Ry S.; Maltinti M.; Vittorini S.; Giannessi D. (2007)
  Matrix metalloprotease-2 and -9 concentration and activity in culture medium and serum samples: methodological evaluation of two different methods
  
  (literal)

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• Colotti C.; Angeli V.; Del Ry S.; Maltinti M.; Vittorini S.; Giannessi D. (literal)

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• Clinical Chemistry and Laboratory Medicine 2007; Vol.45: (W130). (literal)

Convegno

• Clinical Chemistry and laboratory medicine (literal)

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• 45 (literal)

Rivista

• Clinical chemistry and laboratory medicine (Rivista)

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• In: Clinical Chemistry and laboratory medicine, vol. 45 pp. W130 -. S-S473 EUROMEDLAB Amsterdam 2007. Walter de Gruyter, 2007. (literal)

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• Background: Matrix metalloproteases (MMPs) play important roles in many physiopathological conditions charactherized by cardiovascular remodelling possibly due to their degrading action on extracellular matrix. In these conditions, their determination could help in clinical decision making. Aim: To compare two different methods for MMP-2 and -9 determination in human serum samples and culture media from an ex-vivo human model of intimal hyperplasia (IH). Methods: Saphenous vein (SV) segments, obtained from 10 cardiosurgical patients, were cultured for 10 days in RPMI-1640 alone (control) or supplemented with 30% (v/v) FCS (culture) in order to reproduce IH. Human serum samples were obtained from 20 healthy subjects. Total MMP-2 and -9 concentrations were measured by immunometric assays while their activities by enzymatic assays. Results: Both methods were able to detect MMP-2 and -9 with similar sensitivity, reproducibility and accuracy and furnished positively related results, although significantly different, in both kind of samples. MMP-2 in human serum was 544.0±50.3ng/ml (mean±sem) and 182.9±4.8 (p0.001) for enzymatic and immunometric assay, respectively while, MMP-9 was 185.0±18.1 and 429.7±35.1 (p0.001). MMP production in SV culture media increased as a function of IH progression (MMP-2: 173.7±30.0 vs 111.9±26.9ng/ml; MMP-9: 3.8±0.9 vs 2.3±0.6ng/ml, for culture and control, mean±sem). These results were confirmed also at tissue mRNA level. Conclusion: Our data indicate that it is worthy to pay attention to methodological differences when comparing MMP results from different studies because, different concentration values obtained from intrinsically reliable methods, may be due substantially to the differences in assay principles. (literal)

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• CNR IFC (literal)

Titolo

• Matrix metalloprotease-2 and -9 concentration and activity in culture medium and serum samples: methodological evaluation of two different methods (literal)

Prodotto di

• Istituto di fisiologia clinica (IFC) (Istituto)
• Tassonomia clinica molecolare - Tassonomia clinica molecolare (ME.P05.003.001) (Modulo)

Autore CNR

• MARISTELLA MALTINTI (Persona)
• SIMONA VITTORINI (Unità di personale interno)
• DANIELA GIANNESSI (Unità di personale interno)
• SILVIA DEL RY (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Matrix metalloprotease-2 and -9 concentration and activity in culture medium and serum samples: methodological evaluation of two different methods" (Insieme di parole chiave)

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Autore CNR di

• DANIELA GIANNESSI (Unità di personale interno)
• SILVIA DEL RY (Unità di personale interno)
• MARISTELLA MALTINTI (Persona)
• SIMONA VITTORINI (Unità di personale interno)

Prodotto

• Istituto di fisiologia clinica (IFC) (Istituto)
• Tassonomia clinica molecolare - Tassonomia clinica molecolare (ME.P05.003.001) (Modulo)

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• Clinical chemistry and laboratory medicine (Rivista)

Insieme di parole chiave di

• Keywords of "Matrix metalloprotease-2 and -9 concentration and activity in culture medium and serum samples: methodological evaluation of two different methods" (Insieme di parole chiave)