http://www.cnr.it/ontology/cnr/individuo/prodotto/ID10888
Hepatitis C virus nonstructural proteins are localized in a modified endoplasmic reticulum of cells expressing viral subgenomic replicons (Articolo in rivista)
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- Label
- Hepatitis C virus nonstructural proteins are localized in a modified endoplasmic reticulum of cells expressing viral subgenomic replicons (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1006/viro.2001.1229 (literal)
- Alternative label
Mottola G., Cardinali G., Ceccacci A., Trozzi C., Bartholomew L., Torrisi M.R., Pedrazzini E., Bonatti S., Migliaccio G. (2002)
Hepatitis C virus nonstructural proteins are localized in a modified endoplasmic reticulum of cells expressing viral subgenomic replicons
in Virology (N.Y.N.Y., Print)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Mottola G., Cardinali G., Ceccacci A., Trozzi C., Bartholomew L., Torrisi M.R., Pedrazzini E., Bonatti S., Migliaccio G. (literal)
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- http://www.sciencedirect.com/science/article/pii/S0042682201912292 (literal)
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- ISI Web of Science (WOS) (literal)
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- CA, TC, BL, MiG: IRBM \"P. Angeletti,\" Pomezia, Rome, Italy
MoG, BS: Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli \"Federico II,\" Naples, Italy
CG, TMR: Istituto Dermatologico San Gallicano, IRCCS, Rome, Italy
TMR: Dipartimento di Medicina Sperimentale e Patologia, Università di Roma \"La Sapienza,\" Rome, Italy
PE: Consiglio Nazionale delle Ricerche, Centro di Farmacologia Cellulare e Molecolare e Dipartimento di Farmacologia Medica, Università di Milano, Milan, Italy (literal)
- Titolo
- Hepatitis C virus nonstructural proteins are localized in a modified endoplasmic reticulum of cells expressing viral subgenomic replicons (literal)
- Abstract
- For many years our knowledge on hepatitis C virus (HCV) replication has
been based on in vitro experiments or transfection studies. Recently, the
first reliable system for studying viral replication in tissue culture
cells was developed. Taking advantage of this system, we examined in
detail the localization of viral nonstructural (NS) proteins in cells
containing functional replication complexes. By fractionation experiments
and immunomicroscopy, we observed that all NS proteins were associated
with the endoplasmic reticulum (ER) membranes, confirming the hypothesis
that the ER is the site of membrane-associated HCV RNA replication.
Interestingly, NS3 and NS4A were preferentially localized in endoplasmic
reticulum cisternae surrounding mitochondria, suggesting additional
subcellular compartment-related functions for these viral proteins.
Furthermore, the immunoelectron microscopy revealed the loss of the
organization and other morphological alterations of the ER (convoluted
cisternae and paracrystalline structures), resembling alterations observed
in liver biopsies of HCV-infected individuals and in flavivirus-infected
cells. (literal)
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