http://www.cnr.it/ontology/cnr/individuo/prodotto/ID10782
Preparation of starch and soluble sugars of plant material for analysis of carbon isotope composition: a comparison of methods (Articolo in rivista)
- Type
- Label
- Preparation of starch and soluble sugars of plant material for analysis of carbon isotope composition: a comparison of methods (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1002/rcm.4088 (literal)
- Alternative label
Richter A. (1); Wanek W. (1); Werner R. (2); Ghashghaie J. (3); Jäggi M. (4); Gessler A. (5); Brugnoli E. (6);Hettmann E. (7); Göttlicher S.G. (8); Salmon Y. (2); Bathellier C. (3); Kodama N. (9); Nogués S. (10); Soe A. (11); Volders F. (6); Sörgel K (2); Blöchl A (1); Siegwolf R.T.W. (12); Buchmann N. (2); Gleixner G. (7) (2009)
Preparation of starch and soluble sugars of plant material for analysis of carbon isotope composition: a comparison of methods
in RCM. Rapid communications in mass spectrometry; John Wiley & Sons Inc., Hoboken (Stati Uniti d'America)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Richter A. (1); Wanek W. (1); Werner R. (2); Ghashghaie J. (3); Jäggi M. (4); Gessler A. (5); Brugnoli E. (6);Hettmann E. (7); Göttlicher S.G. (8); Salmon Y. (2); Bathellier C. (3); Kodama N. (9); Nogués S. (10); Soe A. (11); Volders F. (6); Sörgel K (2); Blöchl A (1); Siegwolf R.T.W. (12); Buchmann N. (2); Gleixner G. (7) (literal)
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- Conference: 2nd Joint European Stable Isotobe User Meeting (JESIUM) Location: Presquile de Giens, FRANCE Date: AUG 31-SEP 05, 2008 (literal)
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- Rivista
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- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1) Department of Chemical Ecology and Ecosystem Research, University of Vienna, Althanstraße 14, A-1090 Wien, Austria;
2) Institute of Plant Sciences, Universitätsstrasse 2, ETH Zürich LFW C56, 8092 Zürich, Switzerland;
3) Laboratoire d'Ecologie, Systématique et Evolution, CNRS-UMR 8079, Bâtiment 362, Université Paris-Sud (XI), 91405-Orsay cédex, France;
4) Paul Scherrer Institute, Radioanalytics, CH-5232 Villigen PSI, Switzerland;
5) Centre for Systems Biology (ZBSA), Core Facility Metabolomics, University of Freiburg Habsburgerstr. 49, D-79104 Freiburg, Germany;
6) CNR, Institute of Agro-Environmental Biology and Forestry, via Marconi 2, I-05010 Porano, Italy;
7) Research Group Biogeochemical Processes, Max-Planck-Institute for Biogeochemistry, D-07701 Jena, Germany;
8) Department of Forest Ecology and Management, SLU, SE-901 83 Umeå, Sweden;
9) Global Change Processes, Landcare Research, PO Box 40, Gerald Street, Lincoln 7640, New Zealand;
10) Unitat de Fisologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08028, Barcelona, Spain;
11) Fertin Pharma, R&D department, Dandyvej 19, DK-7100 Vejle, Denmark;
12) Paul Scherrer Institute, Laboratory of Atmospheric Chemistry, CH-5232 Villigen PSI, Switzerland (literal)
- Titolo
- Preparation of starch and soluble sugars of plant material for analysis of carbon isotope composition: a comparison of methods (literal)
- Abstract
- Starch and soluble sugars are the major photosynthetic products, and their carbon isotope signatures
reflect external versus internal limitations of CO2 fixation. There has been recent renewed interest in
the isotope composition of carbohydrates, mainly for use in CO2 flux partitioning studies at the
ecosystem level. The major obstacle to the use of carbohydrates in such studies has been the lack of an
acknowledged method to isolate starch and soluble sugars for isotopic measurements. We here report
on the comparison and evaluation of existing methods (acid and enzymatic hydrolysis for starch; ionexchange
purification and compound-specific analysis for sugars). The selectivity and reproducibility
of the methods were tested using three approaches: (i) an artificial leaf composed of a mixture of
isotopically defined compounds, (ii) a C4 leaf spiked with C3 starch, and (iii) two natural plant
samples (root, leaf). Starch preparation methods based on enzymatic or acid hydrolysis did not yield
similar results and exhibited contaminations by non-starch compounds. The specificity of the acidic
hydrolysis method was especially low, and we therefore suggest terming these preparations as HClhydrolysable
carbon, rather than starch. Despite being more specific, enzyme-based methods to
isolate starch also need to be further optimized to increase specificity. The analysis of sugars by ionexchange
methods (bulk preparations) was fast but produced more variable isotope compositions
than compound-specific methods. Compound-specific approaches did not in all cases correctly
reproduce the target values, mainly due to unsatisfactory separation of sugars and background
contamination. Our study demonstrates that, despite their wide application, methods for the
preparation of starch and soluble sugars for the analysis of carbon isotope composition are not
(yet) reliable enough to be routinely applied and further research is urgently needed to resolve the
identified problems. (literal)
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