New curcumin analogues show enhanced antitumour activity in malignant melanoma cells (Abstract/Poster in rivista)

Type
Label
  • New curcumin analogues show enhanced antitumour activity in malignant melanoma cells (Abstract/Poster in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/S1359-6349(08)72212-2 (literal)
Alternative label
  • Marina Pisano, Sara Cossu, Ilaria Sassu, Gabriella Pagnan, Davide Fabbri, Maria Antonietta Dettori, Giuseppe Palmieri, Giovanna Delogu, Mirco Ponzoni and CARLA ROZZO (2008)
    New curcumin analogues show enhanced antitumour activity in malignant melanoma cells
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Marina Pisano, Sara Cossu, Ilaria Sassu, Gabriella Pagnan, Davide Fabbri, Maria Antonietta Dettori, Giuseppe Palmieri, Giovanna Delogu, Mirco Ponzoni and CARLA ROZZO (literal)
Pagina inizio
  • 90 (literal)
Pagina fine
  • 90 (literal)
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  • 6 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 1 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 12 (literal)
Note
  • Abstract (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR, Istituto di Chimica Biomolecolare, sede di Sassari; Istituto G. Gaslini, Laboratorio di Oncologia, Genova. (literal)
Titolo
  • New curcumin analogues show enhanced antitumour activity in malignant melanoma cells (literal)
Abstract
  • Background: Malignant Melanoma (MM) is one of the fastest growing cancer in western populations with the incidence having tripled in the last decades. Chemotherapy, immunotherapy and vaccines are still unsatisfactory thus new approaches for MM treatment are urgently needed. Curcumin, a natural spice extracted from the root of Curcuma longa L. and largely used in oriental cuisine and medicine, has recently been described as potential anticancer agent. We tested several curcumin-related compounds for their capability to inhibit cell growth on primary MM cell lines. Material and methods: Viability and antiproliferative assays together with dose and time-response assays have been carried out on MM cell lines to compare antitumour activity of curcumin to that of six related biphenyls. Cultured fibroblasts from healthy donors have been used as controls. DNA fragmentation with ELISA and TUNEL assays have been performed to asses apoptosis triggered by some of the treatments. Results: Curcumin, a natural compound already known for its antitumor activity, showed to be a potent antiproliferative agent on our MM cells. We tested six curcumin-related hydroxylated biphenyls (D2-D7) on MM cells to assess their potential antitumour activity in comparison with that of curcumin: IC50 values established after 5 days of treatments showed the ?,??unsaturated keton (D6) the most efficient at concentrations around 1-2?M, much lower than the IC50 values calculated for curcumin (about 10?M). Fibroblasts proliferation rate was not affected in the same conditions. Wash-out experiments further demonstrated that the D6 action was more powerful and rapid in arresting MM cells growth than that of curcumin, giving rise to irreversible effects after only 2-4 hours of co-colture with MM cells. Clonogenic assays were performed to measure long-term effects of D6 on permanent cell growth arrest and cell death, showing a dose-dependent reduction in MM colony formation. ELISA and TUNEL assays on some of the MM cell lines allowed the detection of oligonucleosomes in the cytoplasm and apoptotic bodies in the nucleus, showing involvement of apoptosis in D6 activity. Conclusions: Our results indicate this compound as good lead to develop new therapeutic agents against MM. D6 activity should be further investigated on in vivo melanoma models to assess the real anticancer effectiveness on such tumour. (literal)
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