http://www.cnr.it/ontology/cnr/individuo/prodotto/ID103260
UV-B stress induces 14-3-3 epsilon mRNA increase and delocalisation in Paracentrotus lividus sea urchin embryos. (Abstract/Comunicazione in atti di convegno)
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- UV-B stress induces 14-3-3 epsilon mRNA increase and delocalisation in Paracentrotus lividus sea urchin embryos. (Abstract/Comunicazione in atti di convegno) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Alternative label
Russo R, Zito F, Costa C, Bonaventura R, Matranga V. (2010)
UV-B stress induces 14-3-3 epsilon mRNA increase and delocalisation in Paracentrotus lividus sea urchin embryos.
in XXI Convegno Annuale ABCD sul tema Stress cellulare: Sopravvivenza ed Apoptosi, Urbino
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- Russo R, Zito F, Costa C, Bonaventura R, Matranga V. (literal)
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- Istituto di Biomedicina e Immunologia Molecolare, \"Alberto Monroy\", Consiglio Nazionale delle Ricerche, via U.La Malfa 153, 90146, Palermo, Italy (literal)
- Titolo
- UV-B stress induces 14-3-3 epsilon mRNA increase and delocalisation in Paracentrotus lividus sea urchin embryos. (literal)
- Abstract
- UV-B stress induces 14-3-3 epsilon mRNA increase and delocalisation in
Paracentrotus lividus sea urchin embryos
Roberta Russo, Francesca Zito, Caterina Costa, Rosa Bonaventura, Valeria Matranga
Consiglio Nazionale delle Ricerche, Istituto di Biomedicina e Immunologia Molecolare
''Alberto Monroy'', Palermo, Italy
Members of the 14-3-3 protein family are involved in many important cellular
events regulating the equilibrium state between cell survival and apoptosis. They
take part in complex mechanisms involved in signal transduction, transcription,
mitochondrial pathways and others (Morrison 2008). The 14-3-3 genes are highly
conserved, from plants to humans, and some are responsive to UV radiation.
We have previously shown that Paracentrotus lividus (Pl) sea urchin embryos
exposed at cleavage stages to different doses of UV-B, ranging from 10 to 800 J/
m2, develop with dose-dependent morphological abnormalities, including major
defects in skeleton and archenteron (primitive intestine) elongation, and increased
hsp70 protein levels (Bonaventura et al. 2006). It was of interest to investigate the
expression levels and spatial localization of the 14-3-3 gene product (Pl14-3-3
epsilon) in response to UV-B radiation in sea urchin embryos. To this purpose
we isolated the complete cDNA encoding for the 14-3-3 epsilon isoform from
embryos exposed to UV-B at the dose of 800 J/m2. By QPCR we analyzed the
expression levels of the Pl14-3-3 epsilon mRNA during development and found a
stage-dependent proportional increase of the transcript from mesenchyme blastula
to prism. When cleavage stage embryos (32 cells) were exposed to UV-B, we found
a 2.3-fold (400 J/m2) and 2.7-fold (800 J/m2) increase in Pl14-3-3 epsilon mRNA
levels. In addition, we examined the spatial expression of 14-3-3 epsilon mRNA
by in situ hybridization in both control and UV-B exposed embryos harvested
at late developmental stages (pluteus). The transcripts were found restricted to
the archenteron in gastrula stage control embryos, whereas they were widely
delocalised in all germ layers in UV-B exposed embryo. These results paralleled
the failure of archenteron elongation, observed microscopically, and the abnormal
tissue differentiation, detected by specific endoderm markers. In conclusion, this
is the first report describing the complete cDNA coding for the 14-3-3 epsilon
isoform in P. lividus sea urchin embryos and the first evidence of a transcriptional
regulation of the gene in response to UV-B exposure. This work has been supported
in part by: EU - UV-TOX Project, Contract N. EVK3-CT-1999-00005, ASI MoMA
Project Contract N?1/014/06/0, EU- ITN Biomintec Project, Contract N.215507.
Bonaventura R, Poma V, Russo R, Zito F, Matranga V (2006) Effects of UV-B
radiation on development and hsp70 expression in sea urchin cleavage embryos.
Marine Biology 149: 79-86.
Morrison DK (2008) The 14-3-3 proteins: integrators of diverse signaling cues that
impact cell fate and cancer development. Trends in Cell Biology 19: 16-23. (literal)
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