NMR-metabolic methodology in the study of GM foodstuff (Abstract/Poster in atti di convegno)

Type
Label
  • NMR-metabolic methodology in the study of GM foodstuff (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Sobolev A.P., Capitani D., Giannino D., Testone G., Santoro F., Nicolodi C., Iannelli M.A., Mattoo A., Brosio E., Gianferri R., Mannina L. (2009)
    NMR-metabolic methodology in the study of GM foodstuff
    in International Conference on Foodomics, Cesena
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Sobolev A.P., Capitani D., Giannino D., Testone G., Santoro F., Nicolodi C., Iannelli M.A., Mattoo A., Brosio E., Gianferri R., Mannina L. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • 28-29/5/2009 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • The NMR spectroscopy as non specific high- throughput analytical method is a powerful tool to study the metabolic profiling of food either of animal or vegetable origin. The choice of the NMR analytical approach for the food analysis depends on the specific problem. Four different approaches are usually used, namely target analysis, metabolic profiling, metabolomics, metabolic fingerprinting. In our studies a mixed metabolic profiling – metabolomics approach was followed: the NMR spectra of the samples were analyzed and assigned using 2D NMR sequences. Finally, the intensity of the identified metabolites were measured and submitted to a suitable statistical analysis. Although a complete assignment of the NMR spectra are usually performed, we prefer not to define our analytical approach as metabolomics since some metabolites present in extremely low concentrations could be not revealed due to the intrinsic low sensitivity of NMR techniques. Here, we report the results regarding GM tomato and lettuce plants, respectively engineered to accumulate spermidine and asparagine. NMR metabolic profiles assessed that the transgenic lines and the wild-type counterparts shared the same compounds, but they also revealed side effects on the metabolism following the targeted genetic modification. This is an important result for the assessment of the internationally recognized “substantial equivalence”, which measures whether a biotech food shares equivalent nutritional characteristics as its counterpart. In these studied cases, we showed that transgenic crops did not cause the synthesis of novel metabolites, but produced identical metabolites as those of conventional varieties, though in different amounts. (literal)
Titolo
  • NMR-metabolic methodology in the study of GM foodstuff (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Prodotto
Autore CNR di
Insieme di parole chiave di
data.CNR.it