http://www.cnr.it/ontology/cnr/individuo/prodotto/ID102506
Expression of Mybleu gene enhanced tolerance to hypoxic stress in transgenic Carrizo citrange. (Comunicazione a convegno)
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- Expression of Mybleu gene enhanced tolerance to hypoxic stress in transgenic Carrizo citrange. (Comunicazione a convegno) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Alternative label
Caruso P., Pietro Paolo D., Locatelli F., Mattana M., Genga A., Coraggio I., M. R. Paolì, G. Reforgiato Recupero (2009)
Expression of Mybleu gene enhanced tolerance to hypoxic stress in transgenic Carrizo citrange.
in 2nd International Citrus Biotechnology Symposium, Catania
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- Caruso P., Pietro Paolo D., Locatelli F., Mattana M., Genga A., Coraggio I., M. R. Paolì, G. Reforgiato Recupero (literal)
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- November 30 - December 2, 2009 (literal)
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- The Mybleu transcription factor, isolated from rice (a monocot, herbaceous), is able to activate the anaerobic response pathways in Arabidopsis (a dicot, herbaceous). In order to clarify the mechanisms involved in the response to oxygen deficit and to improve tolerance to flooding in citrus species, we overexpressed Mybleu in Carrizo citrange, one of the most popular citrus rootstocks, by Agrobacterium tumefaciens mediated transformation.
In some areas of Italian citrus cultivation the soils is characterized by a content of clay and/or loam in excess determining the inability to eliminate water surplus. In this kind of soils, humidity retention, flooding and/or waterlogging, represent a serious environmental stress that can lead to anaerobic conditions affecting many plant processes.
Analysis by polymerase chain reaction (PCR) and sequencing showed that stable integration of the transgene in the plant genome was obtained in the 71,6% of the analysed plants. Real time PCR performed on a random selection of transgenic plants lines confirmed the expression of the inserted transgene and allowed a classification of the transgenic plants based on the different expression levels of the transgene. Wild type and transgenic lines (low and high expressing) were tested for their tolerance to hypoxia/anoxia. The plants were stressed by flushing under sterile conditions N2. After this treatment the samples were examined by physiological, biochemical and molecular analyses. At the same time, six-month-old transgenic plants both low and highly expressing and wild type were subjected to a flooding treatment by submerging them in a water tank for 11 weeks. The controls untreated were well irrigated and drained. Total RNA extracted from roots of transformed and wild type plants, treated and untreated, were used to hybridise the Combimatrix array.
The results indicated that high expressing plants are more tolerant to hypoxia than wild type and low expressing plants. Mybleu improved tolerance in Carrizo citrange indicates the overexpression of Mybleu may represent a powerful tool to improve hypoxic stress tolerance in agricultural importance species. The hypothesis of a conservation of the regulatory pathways involved in the anaerobic response among different crops is also proposed.
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Caruso P., Pietro Paolo D., Paolì M. R., Reforgiato Recupero G.: CRA-ACM Acireale (CT); Locatelli F., Mattana M., Genga A., Coraggio I.: CNR-IBBA Milano (literal)
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- Expression of Mybleu gene enhanced tolerance to hypoxic stress in transgenic Carrizo citrange. (literal)
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