Development of a microarray technology to SNP detection within milk protein genes in goat (Abstract in rivista)

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Label
  • Development of a microarray technology to SNP detection within milk protein genes in goat (Abstract in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Chessa S., Rignanese D., Raschetti M., Ceriotti G., Caroli A., Pagnacco G., Castiglioni B. (2009)
    Development of a microarray technology to SNP detection within milk protein genes in goat
    in 18° congresso ASPA, Palermo, 2009
    (literal)
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  • Chessa S., Rignanese D., Raschetti M., Ceriotti G., Caroli A., Pagnacco G., Castiglioni B. (literal)
Convegno
  • 18° congresso ASPA (literal)
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  • Palermo (literal)
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  • 9-12/06/2009 L’abstract è stato pubblicato sulla rivista : Italian Journal of Animal Science, vol. 8 (suppl. 2) -2009, pag. 193 (literal)
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  • In the last few years the scientific interest on milk protein genetic polymorphisms in small ruminants has increased because of the effects of same variants on qualitative and technological properties, on biodiversity conservation and, as a consequence, on products typicality. In particular the goat species is characterized by a high genetic variability in casein genes, including 19 variants for aS1-CN with mutation involving at least 7 of the 19 exons of the CSN1S1 gene; 8 alleles and other 3 variants for ²-CN distinguishable by mutations located essentially in exon 7 and 9; 7 alleles for aS2-CN determined by mutations located in four exonic and one intronic region; 16 alleles for º-CN due to 15 mutations all located in exon 4. The analysis of caseins in this species is therefore quite complex and many PCR-derived assays are necessary to have a detailed picture of the genetic variability of an individual. Recently a microarray approach was developed in the bovine species to test simultaneously 22 SNPs in DNA regions responsible for milk protein expression. The method, based on the combination of multiplex PCR (mPCR), ligase detection reaction (LDR) and hybridization onto an universal array (UA), can be an useful tool also for the analysis of goat milk protein variability. In this first version the microarray includes 20 SNPs, which represent a discrete part of the mutations responsible for the listed variants. Further work is necessary to include other polymorphisms and to design a software for the automatic reconstruction of the genotypes. (literal)
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  • SC, DR, GC, GP - VSA-UNIMI; BC, MR - IBBA-CNR; AC – DSBB-UNIBS (literal)
Titolo
  • Development of a microarray technology to SNP detection within milk protein genes in goat (literal)
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