Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A (Abstract/Poster in atti di convegno)

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  • Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Cristoni S., Zingaro L., Canton C., Cremonesi P., Castiglioni B., Morandi S., Brasca M., Luzzana M., Battaglia C. (2009)
    Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A
    in 4th Annual National Conference, Italian Proteomics Association, Milano
    (literal)
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  • Cristoni S., Zingaro L., Canton C., Cremonesi P., Castiglioni B., Morandi S., Brasca M., Luzzana M., Battaglia C. (literal)
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  • 22-25 GIUGNO 2009 (literal)
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  • Surface-activated chemical ionization (SACI) has been widely used in recent years for the analysis of different compounds (e.g., peptides, street drugs, amino acids). The main benefits of this technology are its high sensitivity and its effectiveness under different chromatographic conditions (i.e., ion exchange chromatography and reversed-phase chromatography). We used SACI in conjunction with quadrupole time-of-flight mass spectrometry to analyze enterotoxin A, which is produced by Staphylococcus aureus, in milk matrix using both reversed-phase and ion exchange chromatography. SACI had increased sensitivity as compared with electrospray ionization. Moreover, the higher quantitation efficiency of this technique, mainly in terms of limit of detection (0.01 ng/mL), limit of quantitation (0.05 ng/mL), linearity range (0.05-50 ng/mL), matrix effect, accuracy (intraday and interday accuracy errors were 9.2 and 10.3% respectively) and precision (intraday and interday precision errors were 5.3 and 12.8% respectively), are shown and discussed. (literal)
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  • 1 ISB-Ion Source & Biotechnologies, Milan Italy 2 Insitute of Agricultural Biology and Biotechnology, Italian National Research council, Milan, Italy 3 Insitute of Science and Food Production, Italian National Research council, Milan, Italy 4 Depatment of Biomedical Science and Technology, University of Milan, Italy (literal)
Titolo
  • Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A (literal)
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