http://www.cnr.it/ontology/cnr/individuo/prodotto/ID102489
AtKCO3 potassium channel and tonoplast biogenesis (Abstract/Poster in atti di convegno)
- Type
- Label
- AtKCO3 potassium channel and tonoplast biogenesis (Abstract/Poster in atti di convegno) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Alternative label
Grassi G., Grippa A., Vitale A., Czempinski K., Pedrazzini E. (2009)
AtKCO3 potassium channel and tonoplast biogenesis
in 20th International Conference for Arabidopsis Research (ICAR), Edinburgh, Scotland, UK, 30th June - 4th July 2009
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Grassi G., Grippa A., Vitale A., Czempinski K., Pedrazzini E. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
- Poster Abstract n° P405 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- 30/06/2009 - 04/07/2009 (literal)
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- Abstract: AtKCO3 is a single pore channel with two transmembrane domains and the N- and C-terminal domains exposed in the cytosol. An AtKCO3::GFP fusion was previously found to be located in the tonoplast by transient expression. By subcellular fractionation, we confirmed the tonoplast localization of wild type AtKCO3 and the AtKCO3::GFP fusion in Arabidopsis transgenic plants. We determined that AtKCO3::GFP forms dimers, and not tetramers as predicted, indicating that the C-terminal GFP could interfere with the channel biogenesis. We identified a putative PDZ-binding motif of class 1 (-X-S/T-X-hydrophobic) at the C-terminus of AtKCO3 (-ATSV). PDZ proteins act as adaptors that facilitate signalling or determine localization of various proteins. Preliminary experiments show that deletion of this motif enhances the stability of AtKCO3. This suggests a role of PDZ proteins in determining the turnover and half-life of AtKCO3. Supported by the EU Marie Curie Research Training Network \"Vacuolar Transport Equipment for Growth Regulation in Plants\" (MRTN-CT-2006035833. (literal)
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- GG, AG, AV, EP: IBBA-CNR; KC: University of Potsdam (Germany) (literal)
- Titolo
- AtKCO3 potassium channel and tonoplast biogenesis (literal)
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