Synthesis of the Arabidopsis AtKCO3 potassium channel (Abstract/Poster in atti di convegno)

Type
Label
  • Synthesis of the Arabidopsis AtKCO3 potassium channel (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Grassi G., Grippa A., Vitale A., Czempinski K., Pedrazzini E. (2009)
    Synthesis of the Arabidopsis AtKCO3 potassium channel
    in Società Italiana di Biologia Vegetale. First Congress, Verona
    (literal)
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  • Grassi G., Grippa A., Vitale A., Czempinski K., Pedrazzini E. (literal)
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  • 30/06/2009 - 02/07/2009 (literal)
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  • Abstract: AtKCO3 is a single pore channel with two transmembrane domains and the N- and C-terminal domains exposed in the cytosol. An AtKCO3::GFP fusion was previously found to be located in the tonoplast by transient expression. By subcellular fractionation, we confirmed the tonoplast localization of wild type AtKCO3 and the AtKCO3::GFP fusion in Arabidopsis transgenic plants. We determined that AtKCO3::GFP forms dimers, and not tetramers as predicted, indicating that the C-terminal GFP could interfere with the channel biogenesis. We identified a putative PDZ-binding motif of class 1 (-X-S/T-X-hydrophobic) at the C-terminus of AtKCO3 (-ATSV). PDZ proteins act as adaptors that facilitate signalling or determine localization of various proteins. Preliminary experiments show that deletion of this motif enhances the stability of AtKCO3. This suggests a role of PDZ proteins in determining the turnover and half-life of AtKCO3. Supported by the EU Marie Curie Research Training Network \"Vacuolar Transport Equipment for Growth Regulation in Plants\" (MRTN-CT-2006–035833). (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • GG, AG, AV, EP: IBBA-CNR; KC: University of Potsdam (Germany) (literal)
Titolo
  • Synthesis of the Arabidopsis AtKCO3 potassium channel (literal)
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