Know what you print, to know what you eat (Abstract/Poster in atti di convegno)

Type
Label
  • Know what you print, to know what you eat (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Chiusa I. Morello L., Breviario D. (2009)
    Know what you print, to know what you eat
    in International Conference FOOD-OMICS: A Science for Nutrition, Health and Wellness in the Post-Genomic Era, Cesena
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Chiusa I. Morello L., Breviario D. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • 28-29 maggio 2009 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Food/feed quality, safety and composition has become an issue of the up most relevance world-wide. Accordingly, there is now a great demand for technologies and tools that can easily, rapidly, conveniently and reliably identify plant species and varieties to certify quality and safety while combating frauds. In order to meet with this request we have recently developed a method for plant species recognition that is based on the Microcontact Printing technique [1,2]. This is a new method by which highly organized structures, such as the tracks of a compact disk, can be used to produce stamps on which a diagnostic molecule (i.e. a short DNA sequence) can be inked. The stamps are then used to print a small surface that then becomes the site of molecular recognition carried out through hybridization methods. The advantages of such a technique are multiple: recognition reactions are carried out at microscale level with the use of a limited amounts of reagents and target molecules, kits made up by multiple target molecules useful for food diagnosis can be produced, diagnostic efficiency is further increased by multiple replicas obtained from the same stamp. We have successfully worked out this technology by printing plant PCR amplification products that are selectively recognized through hybridization with specific fluorescently labelled oligonucleotide probes. The assay, granting species selective recognition, was first developed on rice but its application to the identification of small amounts of undesired plant components in food (i.e allergenic species) is under way. The system was also applied to the identification of DNA of plant origin in DNA isolated from goat milk, through selective hybridization of microprinted PCR reactions (see posters of Ponzoni et al and Mastromauro et al). Yet, this is just the beginning of future developments in the field of plant species recognition in feed or food products (i.e. milk or cheese). [1] S. A. Lange, V. Benes, D. P. Kern, J. K. H. Hörber and A. Bernard (2004) Microcontact Printing of DNA molecules. Analytical Chemistry 76. 1641-1647. [2] C. Thibault, V. Le Berre, S. Casimirius, E. Trévisiol, J. François and C. Vieu (2005) Direct microcontact printing of oligonucleotides for biochip applications. Journal of Nanobiotechnology 3: 7. (literal)
Titolo
  • Know what you print, to know what you eat (literal)
Prodotto di
Autore CNR

Incoming links:


Prodotto
Autore CNR di
data.CNR.it