Bean genomic polymorphism uncovered by a systematic approach based on ²-tubulin gene sequence organization. (Abstract/Poster in atti di convegno)

Type
Label
  • Bean genomic polymorphism uncovered by a systematic approach based on ²-tubulin gene sequence organization. (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Braglia L., Ponzoni E., Breviario D. (2009)
    Bean genomic polymorphism uncovered by a systematic approach based on ²-tubulin gene sequence organization.
    in International Conference FOOD-OMICS: A Science for Nutrition, Health and Wellness in the Post-Genomic Era, Cesena
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Braglia L., Ponzoni E., Breviario D. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • 28-29 maggio 2009 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Common bean (Phaseolus vulgaris L.) is the most widely consumed grain legume in the world. Among the five domesticated species, P. vulgaris is the major source of protein for rural and urban populations in Latin America and eastern Africa. Its balance of essential amino acids in seeds is complementary to that in grains of cereals, providing a positive healthy contribute. In Italy, different local ecotypes are known, cultivated in small areas with traditional cropping practices and reported to provide an important yet differential nutritional contribution. However, traditional breeding programs are limited by the under-utilization of the available genetic diversity and the evaluation of phenotypic differences remains the method for evolutionary and pedigree relationship determination. Advances in molecular biology have allowed the development of rapid, sensitive and specific screening methods to study genetic diversity and relatedness between plant species and varieties. One recently developed, useful genotyping method is based on intron length polymorphisms present in members of the plant ²-tubulin gene family (cTBP). The genomic organization of these genes, that encode proteins of relevance for growth, is such to allow a multiple approach for detection of genetic diversity. In addition to the classic cTBP method, we introduce a new approach that uncovers gene-sequence variability in the 5’upstream region of the beta-tubulin genes. Applied on different bean ecotypes, this AFLP like technique holds the potential to become a new powerful DNA fingerprinting tool for studying genetic relationships and diversity. (literal)
Titolo
  • Bean genomic polymorphism uncovered by a systematic approach based on ²-tubulin gene sequence organization. (literal)
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