http://www.cnr.it/ontology/cnr/individuo/prodotto/ID102474
Phenotypic and genotypic aspects of Staphylococcus aureus isolated from chronic subclinical infections in dairy goats. (Abstract in rivista)
- Type
- Label
- Phenotypic and genotypic aspects of Staphylococcus aureus isolated from chronic subclinical infections in dairy goats. (Abstract in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Paolo Moroni; Giuliano Pisoni; Chiara Vimercati; Micaela Antonini; Bianca Castiglioni; Paola Cremonesi; Paul Boettcher; (literal)
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Paolo Moroni; Giuliano Pisoni; Chiara Vimercati; Micaela Antonini - DIPAV-UNIMI;
Bianca Castiglioni; Paul Boettcher - IBBA-CNR;
Paola Cremonesi - DISTEB-UNIMI; (literal)
- Titolo
- Phenotypic and genotypic aspects of Staphylococcus aureus isolated from chronic subclinical infections in dairy goats. (literal)
- Abstract
- The objectives of this study were to identify goats with chronic infections by
Staphylococcus aureus (SAUR), isolate bacteria from these animals, genotype
the bacteria to identify different strains, and perform tests of antimicrobial sensitivity.
A herd of 75 Alpine goats in Northern Italy was monitored for a complete
production season. Bacterial cultures were taken from each udder half
during eight monthly visits. Goats with at least 2 consecutive positive tests for
SAUR (n = 28) in the same udder half were identified as chronically infected.
Goats with no infections in either udder half during any visit were considered
healthy (n = 26). The bacteria isolated from one sample from each infected goat
were genotyped based on variable numbers of tandem repeats in 6 genomic
regions. One sample from each animal was also subject to a test for betalactamase
production and to Minimum Inhibitory Concentration tests for 11
antimicrobial agents: benzylpenicillin, ampicillin, amoxicillin, amoxicillin plus
clavulanic acid, cloxacillin, cephalonium, cephoperazone, oxytetracycline,
doxicycline, kanamycin, and lincomicin. A linear mixed model was used to
examine the relationship between chronic infection by SAUR and somatic cell
score. This analysis involved 841 records. Factors in the statistical model were
sample day, parity, infection status, goat, and udder half nested within goat. No
genetic variability was observed among the bacteria isolated, suggesting that
all were from the same strain. All SAUR isolates were negative for the betalactamase
production test and no isolate was resistant to any of the antimicrobial
agents used. Penicillins were the most effective drugs tested, however. As
expected, SCS was significantly higher in infected goats (least-square mean =
7.55) than in healthy goats (LSM = 5.50). With regard to specific udder halves,
mean SCS from infected udder halves (LSM = 8.02) was greater than in
uninfected udder halves from the same goats (LSM = 6.44). No differences
were observed in milk yield or fat and protein percentages between infected
and healthy goats. (literal)
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