A proteomic analysis of changes in prothrombin and plasma proteins associated with the G20210A mutation (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A proteomic analysis of changes in prothrombin and plasma proteins associated with the G20210A mutation (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Alternative label

• Gelfi C. 1, Vigano A.. 1, Ripamonti M. 1, Wait R. 2, Begum S. 2, Biguzzi E. 3, Castaman G. 4, Faioni E.M. 5 (2004)
  A proteomic analysis of changes in prothrombin and plasma proteins associated with the G20210A mutation
  in Proteomics (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Gelfi C. 1, Vigano A.. 1, Ripamonti M. 1, Wait R. 2, Begum S. 2, Biguzzi E. 3, Castaman G. 4, Faioni E.M. 5 (literal)

Pagina inizio

• 2151 (literal)

Pagina fine

• 2159 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 7 (literal)

Rivista

• Proteomics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Corresponding author: C. Gelfi (cecilia.gelfi@ibfm.cnr.it) (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Institute of Molecular Bioimaging and Physiology, CNR, Segrate, Milano, Italy 2. Kennedy Institute of Rheumatology, Imperial College London, UK 3. Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Department of Internal Medicine, Milano, Italy 4. Division of Hematology, Ospe San Bortolo, Vicenza, Italy 5. Hematology and Thrombosis Unit, DMCO-University of Milano and Ospedale San Paolo, Milano, Italy (literal)

Titolo

• A proteomic analysis of changes in prothrombin and plasma proteins associated with the G20210A mutation (literal)

Abstract

• The G-->A mutation at position 20210 of the prothrombin gene, localized in the 3'-polyadenylation untranslated region of the mRNA, is a recognized genetic risk factor for venous thromboembolism. The mechanism by which this base change confers an increased risk of thrombosis compared to noncarriers is undefined. Studies on the mRNA suggest enhanced cleavage site recognition and a change in the location of the 3'-cleavage/polyadenylation reaction, but no defined model has been proposed. The present study, based on proteomic investigation by two-dimensional gel electrophoresis and electrospray ionization (ESI) tandem mass spectrometry (MS/MS) protein identification, suggests that the G20210A mutation is associated with increased glycosylation of prothrombin, which confers greater stability to the protein. Additionally, proteomic investigation of pooled plasma showed that expression levels of six spots, three of them identified by ESI MS/MS, were altered in subjects carrying the mutation, suggesting a possible cooperative effect in the thrombotic risk increment induced by the mutation. (literal)

Prodotto di

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)

Autore CNR

• MARILENA RIPAMONTI (Persona)
• CECILIA GELFI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "A proteomic analysis of changes in prothrombin and plasma proteins associated with the G20210A mutation" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CECILIA GELFI (Unità di personale interno)
• MARILENA RIPAMONTI (Persona)

Prodotto

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Proteomics (Rivista)

Insieme di parole chiave di

• Keywords of "A proteomic analysis of changes in prothrombin and plasma proteins associated with the G20210A mutation" (Insieme di parole chiave)