http://www.cnr.it/ontology/cnr/individuo/prodotto/ID10148
Preferential counterion binding to A-tract DNA oligomers. (Articolo in rivista)
- Type
- Label
- Preferential counterion binding to A-tract DNA oligomers. (Articolo in rivista) (literal)
- Anno
- 2001-01-01T00:00:00+01:00 (literal)
- Alternative label
Stellwagen N.C., Magnusdottir S., Gelfi C., Righetti P.G. (2001)
Preferential counterion binding to A-tract DNA oligomers.
in Journal of Molecular Biology
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- Stellwagen N.C., Magnusdottir S., Gelfi C., Righetti P.G. (literal)
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- Titolo
- Preferential counterion binding to A-tract DNA oligomers. (literal)
- Abstract
- The free solution mobility of four 20 bp DNA oligomers, with and without A-tracts, has been measured by capillary electrophoresis in Tris-acetate buffer, to test the hypothesis that site-specific binding of monovalent counterions can occur in the narrow minor groove of A-tract DNAs. Preferential counterion binding has been proposed to cause A-tract bending because of asymmetric charge neutralization and collapse of the helix backbone toward the minor groove. Preferential counterion binding in A-tract DNAs should be manifested by a decrease in the electrophoretic mobility observed in free solution, compared to that of non-A-tract DNAs of the same size. Of the four sequences studied here, the slowest absolute mobility, indicative of the greatest counterion binding, was observed for a 20 bp oligomer containing two runs of A3T3 in phase with the helix repeat. A 20-mer containing phased CACA sequences migrated with the fastest mobility; 20-mers containing phased A5 tracts or phased runs of T3A3 migrated with intermediate mobilities. Very similar mobility differences were observed when 1-20 mM NaCl was added to the buffer. The results suggest that preferential counterion binding occurs in A-tract DNAs, especially those containing the AnTn sequence motif.
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