Modulation by Zn2+ and Cd2+ of GABAA receptors of rat cerebellum granule cells in culture (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Modulation by Zn2+ and Cd2+ of GABAA receptors of rat cerebellum granule cells in culture (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Alternative label

• Casagrande S., Valle L., Cupello A., Robello M. (2003)
  Modulation by Zn2+ and Cd2+ of GABAA receptors of rat cerebellum granule cells in culture
  in European biophysics journal
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Casagrande S., Valle L., Cupello A., Robello M. (literal)

Pagina inizio

• 40 (literal)

Pagina fine

• 46 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 32 (literal)

Rivista

• European biophysics journal (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Modulation by Zn2+ and Cd2+ of GABAA receptors of rat cerebellum granule cells in culture (literal)

Abstract

• This study aims to characterize more closely the different populations of GABA(A) receptors present on the cerebellar granule cells of the rat. The effects of two divalent cations, Zn(2+) and Cd(2+), on GABA-activated chloride currents were studied using the whole-cell patch-clamp technique. Zinc cations inhibit differently the peak and the steady-state current elicited by 10 micro M GABA. In fact, Zn(2+) appears to be more potent in inhibiting the steady-state component, with a lower IC(50). The inhibition of the peak component is of the competitive type, whereas the inhibition of the steady-state one is mixed, being partly competitive and partly allosteric. In addition, Cd(2+) has an inhibitory effect on GABA-activated chloride currents. In terms of the peak component, its effect is limited in extent with a maximal inhibition of only 26%, but with a high affinity (IC(50) as low as 0.03 micro M). The steady-state component is inhibited by 20% independently from the Cd(2+) concentration, in the 10(-2)-10(2) micro M range. In this case, the inhibitory mechanism appears to be of the competitive type for the peak component and of the allosteric type for the steady-state one. We suggest these data are a further confirmation that the rapidly and slowly desensitizing components of the GABA-activated chloride currents, corresponding respectively to the peak and the steady-state components, are made up of two different receptor populations. (literal)

Prodotto di

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)

Autore CNR

• AROLDO CUPELLO (Unità di personale interno)

Incoming links:

Prodotto

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)

Autore CNR di

• AROLDO CUPELLO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European biophysics journal (Rivista)