Immunocytochemical study of a1 and ß2/3 subunits of GABAA receptors in free-hand isolated vestibular Deiters' neurons (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Immunocytochemical study of a1 and ß2/3 subunits of GABAA receptors in free-hand isolated vestibular Deiters' neurons (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Alternative label

• Rapallino M.V., Cupello A., Luccardini C., Nieddu E., Seitun A., Robello M. (2003)
  Immunocytochemical study of a1 and ß2/3 subunits of GABAA receptors in free-hand isolated vestibular Deiters' neurons
  in Receptors & channels (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Rapallino M.V., Cupello A., Luccardini C., Nieddu E., Seitun A., Robello M. (literal)

Pagina inizio

• 77 (literal)

Pagina fine

• 81 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 9 (literal)

Rivista

• Receptors & channels (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Immunocytochemical study of a1 and ß2/3 subunits of GABAA receptors in free-hand isolated vestibular Deiters' neurons (literal)

Abstract

• Vestibular Deiters' neurons have been isolated from bovine brain by the Hyden's freehand dissection technique and challenged with monoclonal antibodies directed toward the alpha 1 and beta 2/3 subunits of the GABAA receptors. Subsequent challenge with fluorescent secondary antibodies and confocal microscopy allowed the study of the cellular distribution of such subunits. In Deiters' neurons the beta 2/3 subunit displayed a clear presence all along the cell body profile and the initial parts of the dendrites. The alpha 1 subunit was found highly present all over the cell interior except the nuclear profiles. The strong presence inside the cells possibly masked its presence on the plasma membrane. However, in part of the cells studied a distinct presence on the plasma membrane was evident. This subunit was visualized also all along the long dendrites of these neurons. The approach we describe here, involving freehand isolated mature neurons from adult animals, may allow a better characterization of the tridimensional distribution of different types of neuronal GABAA receptors in the respect of the approach with brain slices. (literal)

Prodotto di

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)

Autore CNR

• AROLDO CUPELLO (Unità di personale interno)

Incoming links:

Prodotto

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)

Autore CNR di

• AROLDO CUPELLO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Receptors & channels (Rivista)