Progetto AIRC: MiRNAs in tumorigenesis: novel oncosuppressor targets and miRNA-mediated control of AP-1 transcription factors (SV.P15.007.002)

Type
Label
  • Progetto AIRC: MiRNAs in tumorigenesis: novel oncosuppressor targets and miRNA-mediated control of AP-1 transcription factors (SV.P15.007.002) (literal)
Codice
  • SV.P15.007.002 (literal)
Anno di chiusura previsto
  • 2013-01-01T00:00:00+01:00 (literal)
Istituto esecutore
Abstract
  • Several tumor-associated miRNAs have been characterized as components of regulatory feedback loops involving oncogenic and oncosuppressor transcription factors, such as Myc, NF-kB and p53. MiRNAs, in turn, can control the synthesis of their own transactivators, by both direct and indirect mechanisms forming complex regulatory networks. The AP-1 transcription factors are key targets of multiple tumorigenic pathways. The oncogenic role of AP-1 is complex, because of the large number of homo- and hetero- dimers formed by JUN and FOS family members, which play distinct, sometimes antagonistic functions, depending on the cell-context. Recently, in an in vitro system of thyroid cell transformation, we have reported that the miR-21 precursor (pri-miR-21) is transcriptionally regulated by AP-1 in response to the RAS oncoprotein. In this project, we will: - identify novel tumor suppressor targets of miR-21 in multiple tumor cell lines; - characterize the mechanisms of miRNA-mediated regulation of various AP-1 components in neoplastic transformation; - adopt high-throughput approaches, based on AP-1-regulated reporters as readouts for functional screening of miRNAs affecting AP-1 activity. (literal)
Nome
  • Progetto AIRC: MiRNAs in tumorigenesis: novel oncosuppressor targets and miRNA-mediated control of AP-1 transcription factors (literal)
Descrizione
Modulo di
Gestore

Incoming links:


Modulo
Istituto esecutore di
Descrizione di
Gestore di
data.CNR.it